动态增强MRI用于检测鼻咽癌肿瘤内乏氧和血管生成的研究
发布时间：2019-06-10 13:30
【摘要】： 目的探讨鼻咽癌MRI动态增强参数与微血管密度(MVD)和乏氧诱导因子-1α(HIF-α)、血管内皮生长因子(VEGF)表达的相关性。以了解Gd-DTPA动态增强MRI对诊断肿瘤内乏氧和微血管情况的有效性。 方法①14例鼻咽癌初诊患者,在鼻咽纤维镜下标记新生物中一区域为研究感兴趣区(ROI),行MR动态增强扫描,获取该区域动态增强曲线；然后对标记处进行鼻咽镜活检,测定活检部位的MVD和HIF-1α、VEGF表达水平；②观察动态增强曲线特征,测量ROI的MRI动态增强半定量参数对比剂到达肿瘤组织时间(time at arrival of contrast inflow, Tl on set)、到达强化高峰时间(time to peak, TTP)、强化曲线平均斜率及最大斜率(slope and maximum slope,MS)、最大信号强度值(maximum of signal indensity, SImax),并计算ROI的MRI动态增强定量参数血浆与EES间的容积转移常量(Ktrans)、血浆与EES间的速率常数(kep)、EES容积(渗漏空间,ve)；③对各参数值与MVD和HIF-1α、VEGF表达水平进行相关性分析。 结果ROI的半定量参数slope、MS、SImax与VEGF的表达呈正相关关系(r分别为0.889,0.882,0.619,p0.05),与HIF-1α的表达呈正相关关系(r分别为0.870,0.825,0.665,p0.05),与MVD也呈正相关关系(r分别为0.627,0.755,0.693,p0.05); T1 on set.TTP与VEGF表达呈负相关关系(r分别为-0.909,-0.829,p0.05),与HIF-1α表达呈负相关关系(r分别为-0.687,-0.690,p0.05),与MVD也呈负相关关系(r分别为-0.578,-0.852,p0.05) ROI的定量参数Ktrans、kep与VEGF的表达呈正相关关系(r分别为0.540,0.691,p0.05),与HIF-1α的表达呈正相关关系(r分别为0.536,0.677,p0.05)；kep与MVD也呈正相关关系(r为0.635, p0.05); Ktrans与MVD无明显统计学相关性；ve与VEGF、HIF-1α表达均呈负相关关系(r分别为-0.624,-0.595,p0.05),与MVD也呈负相关关系(r为-0.667,p0.05) 结论鼻咽癌活检部位的MRI动态增强半定量、定量参数与其MVD和HIF-1α、VEGF的表达有统计学相关性；MR动态增强扫描技术具有提供鼻咽癌肿瘤内乏氧与血管生成信息的能力。
[Abstract]:Objective to investigate the correlation between dynamic enhancement parameters of MRI and the expression of microvessel density (MVD), hypoxia inducible factor-1 伪 (HIF- 伪) and vascular endothelial growth factor (VEGF) in nasopharyngeal carcinoma (NPC). To investigate the effectiveness of Gd-DTPA dynamic contrast-enhanced MRI in the diagnosis of hypoxia and microvessels in tumors. Methods MR dynamic enhancement scan was performed in 114patients with nasopharyngeal carcinoma (NPC) in order to study the region of interest (ROI), and the dynamic enhancement curve of the region was obtained by labeling a region of interest in the new organism under nasopharyngeal fiberscope. Then nasopharyngeal biopsy was performed to detect the expression of MVD, HIF-1 伪 and VEGF in the biopsies. (2) the characteristics of dynamic enhancement curve were observed, and the MRI dynamic enhancement semi-quantitative parameters of ROI were measured. The time (time at arrival of contrast inflow, Tl on set), reached the peak of enhancement (time to peak, TTP),. The mean slope and maximum slope of enhancement curve (slope and maximum slope,MS), the maximum signal intensity value (maximum of signal indensity, SImax), and the MRI dynamic enhancement quantitative parameters of ROI were calculated. The volume transfer constant (Ktrans), between plasma and EES was calculated. Rate constant (kep), EES volume between plasma and EES (leakage space, ve);) (3) the correlation between each parameter value and the expression level of MVD, HIF-1 伪 and VEGF was analyzed. Results the semi-quantitative parameter slope,MS,SImax of ROI was positively correlated with the expression of VEGF (r = 0.889, 0.882, 0.619, p0.05) and with the expression of HIF-1 伪 (r = 0.870, 0.825, 0.665, p0.05, respectively). There was also a positive correlation with MVD (r = 0. 627, 0. 755, 0. 693, p0.05). The expression of T1 on set.TTP was negatively correlated with the expression of VEGF (r =-0.909, 鈮，
本文编号：2496489
[Abstract]:Objective to investigate the correlation between dynamic enhancement parameters of MRI and the expression of microvessel density (MVD), hypoxia inducible factor-1 伪 (HIF- 伪) and vascular endothelial growth factor (VEGF) in nasopharyngeal carcinoma (NPC). To investigate the effectiveness of Gd-DTPA dynamic contrast-enhanced MRI in the diagnosis of hypoxia and microvessels in tumors. Methods MR dynamic enhancement scan was performed in 114patients with nasopharyngeal carcinoma (NPC) in order to study the region of interest (ROI), and the dynamic enhancement curve of the region was obtained by labeling a region of interest in the new organism under nasopharyngeal fiberscope. Then nasopharyngeal biopsy was performed to detect the expression of MVD, HIF-1 伪 and VEGF in the biopsies. (2) the characteristics of dynamic enhancement curve were observed, and the MRI dynamic enhancement semi-quantitative parameters of ROI were measured. The time (time at arrival of contrast inflow, Tl on set), reached the peak of enhancement (time to peak, TTP),. The mean slope and maximum slope of enhancement curve (slope and maximum slope,MS), the maximum signal intensity value (maximum of signal indensity, SImax), and the MRI dynamic enhancement quantitative parameters of ROI were calculated. The volume transfer constant (Ktrans), between plasma and EES was calculated. Rate constant (kep), EES volume between plasma and EES (leakage space, ve);) (3) the correlation between each parameter value and the expression level of MVD, HIF-1 伪 and VEGF was analyzed. Results the semi-quantitative parameter slope,MS,SImax of ROI was positively correlated with the expression of VEGF (r = 0.889, 0.882, 0.619, p0.05) and with the expression of HIF-1 伪 (r = 0.870, 0.825, 0.665, p0.05, respectively). There was also a positive correlation with MVD (r = 0. 627, 0. 755, 0. 693, p0.05). The expression of T1 on set.TTP was negatively correlated with the expression of VEGF (r =-0.909, 鈮，
本文编号：2496489
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