机械拉伸对兔角膜成纤维细胞增殖和迁移的影响

发布时间：2019-06-27 20:55

【摘要】：对兔角膜成纤维细胞施加5%应变、0.1Hz的周期性机械拉伸,采用流式细胞仪检测细胞增殖情况,划痕法研究细胞迁移情况。结果发现,与静态对照组相比,5%机械拉伸能够使S期细胞数量明显增多;施加1ng/mL表皮生长因子(Epidermal growth factor,EGF)或100ng/mL碱性成纤维细胞生长因子(Basic fibroblast growth factor,bFGF)后细胞相对划痕宽度明显变小。与各自静态对照组相比,拉伸10h,单轴拉伸组相对划痕宽度明显增加,等双轴拉伸则无显著性差异;拉伸20h,无论是等双轴还是单轴拉伸,相对划痕宽度均明显增加;拉伸同时施加bFGF或EGF后相对划痕宽度均减小,与静态对照组相当。提示机械拉伸能够促进角膜成纤维细胞增殖,但抑制了细胞迁移行为,该抑制效应与拉伸作用方式有关;而bFGF和EGF则可以减弱牵拉引起的细胞迁移抑制效应。
[Abstract]:Rabbit corneal fibroblasts were subjected to 5% strain and periodic mechanical stretching of 0.1Hz. Cell proliferation was detected by flow cytometry and cell migration was studied by scratch method. The results showed that compared with the static control group, 5% mechanical stretching could significantly increase the number of S phase cells, and the relative scratch width of S phase cells decreased significantly after the application of 1ng/mL epidermis growth factor (Epidermal growth factor,EGF or 100ng/mL basic fibroblast growth factor (Basic fibroblast growth factor,bFGF). Compared with the static control group, the relative scratch width of the uniaxial drawing group increased significantly at 10 h, but there was no significant difference between the uniaxial tension group and the equal biaxial tension group, and the relative scratch width of the uniaxial tension group increased significantly after stretching for 20 h, and the relative scratch width decreased after stretching with bFGF or EGF at the same time, which was similar to that of the static control group. It is suggested that mechanical stretching can promote the proliferation of corneal fibroblasts, but inhibit the migration behavior of corneal fibroblasts, which is related to the way of stretching, while bFGF and EGF can weaken the inhibitory effect of cell migration induced by traction.
【作者单位】：太原理工大学应用力学与生物医学工程研究所;
【基金】：国家自然科学基金资助项目(11032008) 山西省自然科学基金资助项目(2012011046-2)
【分类号】：R772

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