鳞癌抗原在鼻内翻乳头状瘤组织中的表达及临床意义
发布时间：2019-07-06 16:23
【摘要】：研究背景与目的鼻内翻乳头状瘤(sinonasal inverted papilloma, NIP)是一种发生于鼻腔及鼻窦粘膜上皮的良性增生性病变,其典型的组织病理学特征为复层上皮向内翻转长入间质组织中。临床上,NIP主要面临三个问题:局部侵袭,复发倾向及恶变倾向。因而,治疗上除彻底手术切除外,还应进行长期术后随访。鳞癌抗原(squamous cell carcinoma antigen, SCCA)是一种肿瘤相关蛋白,它最早被发现于宫颈癌组织中。目前, SCCA作为一种鳞癌相关实验室指标被广泛应用。近来,有研究发现NIP患者的血清SCCA水平明显增高,并且其术后血清SCCA水平较术前明显下降。因此,SCCA有可能作为一种针对NIP有用的生物学指标加以应用。本次实验的目的在于研究SCCA在NIP组织中的表达情况,评估SCCA作为NIP相关的生物学指标加以临床应用的可能性。 材料与方法采用免疫组化对31例NIP标本、15例鼻息肉(nasal polyp, NP)标本及8例鼻腔鳞癌(nasal squamous cell carcinoma, NSCC)标本进行SCCA单克隆抗体标记染色。所有标本均来自安徽省立医院耳鼻喉科术后病理标本,标本经10%福尔马林溶液固定,常规石蜡包埋,采用4μm切片厚度连续切片。 结果所有标本均可见SCCA免疫反应阳性。对比NIP标本与NP标本、NSCC标本的染色,可见NIP标本的染色明显强于NP标本及NSCC标本。其染色强度等级差异有统计学意义(NIP与NP标本对比χ~2= 16.847,P0.01;NIP与NSCC标本对比χ~2= 4.648;P0.05)。在NIP标本中,大多数标本的染色部位在基底层之上的上皮细胞,其基底层细胞染色为阴性,但在3例术后随访发现恶变的病例,其标本基底层染色均为阳性。恶变NIP标本与无恶变NIP标本的基底层染色率对比,其差异有统计学意义(χ~2 =11.394; P0.05)。 结论本研究显示,SCCA在NIP标本中有强阳性表达,这表明SCCA在NIP的发生发展中可能起到一定的作用。临床上,对SCCA的检测可用作NIP诊断治疗及术后随访的一项有用的生物学指标。NIP恶变病例基底层细胞SCCA染色均为阳性,临床上可考虑将SCCA基底层染色作为判断NIP恶变倾向的指标。
文内图片：
图片说明：NIP常规HE染色×100Fig1：NIP，H&Estain×100
[Abstract]:Background and objective (sinonasal inverted papilloma, NIP) is a benign hyperplastic lesion occurring in the mucosa of nasal cavity and paranasal sinus. its typical histopathological feature is that the lamellar epithelial layer is turned inward into the stroma. Clinically, NIP mainly faces three problems: local invasion, recurrence tendency and malignant transformation tendency. Therefore, in addition to thorough surgical resection, long-term postoperative follow-up should be carried out. Squamous cell carcinoma antigen (squamous cell carcinoma antigen, SCCA) is a tumor-associated protein, which was first found in cervical cancer. At present, SCCA is widely used as a laboratory index related to squamous cell carcinoma. Recently, it has been found that the level of serum SCCA in patients with NIP is significantly higher than that before operation, and the level of serum SCCA after operation is significantly lower than that before operation. Therefore, SCCA may be used as a useful biological index for NIP. The purpose of this study was to study the expression of SCCA in NIP and to evaluate the possibility of clinical application of SCCA as a biological index related to NIP. Materials and methods Immunohistochemical staining of 31 cases of NIP, 15 cases of nasal polyp (nasal polyp, NP) and 8 cases of nasal squamous cell carcinoma (nasal squamous cell carcinoma, NSCC) were stained with SCCA monoclonal antibody. All the specimens were collected from the department of otolaryngology, Anhui Provincial Hospital. The specimens were fixed with 10% formalin solution and embedded in paraffin. The specimens were sectioned continuously with 4 渭 m slice thickness. Results all the specimens were positive for SCCA. Comparing the staining of NIP specimens with NP specimens and NSCC specimens, it can be seen that the staining of NIP specimens is significantly stronger than that of NP specimens and NSCC specimens. There was significant difference in staining intensity between NIP and NP (蠂 ~ 2 = 16.847, P < 0.01 / nip and NSCC, P ~ 2 = 4.648, P < 0.05). In NIP specimens, most of the specimens were stained in the epithelial cells above the basal layer, and the basal cells were negative, but in 3 cases of malignant transformation, the staining of the basal layer of the specimens was positive. There was significant difference in the staining rate of basal layer between malignant NIP specimens and non-malignant NIP specimens (蠂 ~ 2 = 11.394; P 0.05). Conclusion SCCA is strongly positive in NIP samples, which indicates that SCCA may play a role in the occurrence and development of NIP. Clinically, the detection of SCCA can be used as a useful biological index for the diagnosis, treatment and postoperative follow-up of NIP. SCCA staining of basal cells in nip malignant transformation cases is positive, and SCCA basal staining can be considered as an index to judge the malignant transformation tendency of NIP.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R739.62
本文编号：2511162
文内图片：
图片说明：NIP常规HE染色×100Fig1：NIP，H&Estain×100
[Abstract]:Background and objective (sinonasal inverted papilloma, NIP) is a benign hyperplastic lesion occurring in the mucosa of nasal cavity and paranasal sinus. its typical histopathological feature is that the lamellar epithelial layer is turned inward into the stroma. Clinically, NIP mainly faces three problems: local invasion, recurrence tendency and malignant transformation tendency. Therefore, in addition to thorough surgical resection, long-term postoperative follow-up should be carried out. Squamous cell carcinoma antigen (squamous cell carcinoma antigen, SCCA) is a tumor-associated protein, which was first found in cervical cancer. At present, SCCA is widely used as a laboratory index related to squamous cell carcinoma. Recently, it has been found that the level of serum SCCA in patients with NIP is significantly higher than that before operation, and the level of serum SCCA after operation is significantly lower than that before operation. Therefore, SCCA may be used as a useful biological index for NIP. The purpose of this study was to study the expression of SCCA in NIP and to evaluate the possibility of clinical application of SCCA as a biological index related to NIP. Materials and methods Immunohistochemical staining of 31 cases of NIP, 15 cases of nasal polyp (nasal polyp, NP) and 8 cases of nasal squamous cell carcinoma (nasal squamous cell carcinoma, NSCC) were stained with SCCA monoclonal antibody. All the specimens were collected from the department of otolaryngology, Anhui Provincial Hospital. The specimens were fixed with 10% formalin solution and embedded in paraffin. The specimens were sectioned continuously with 4 渭 m slice thickness. Results all the specimens were positive for SCCA. Comparing the staining of NIP specimens with NP specimens and NSCC specimens, it can be seen that the staining of NIP specimens is significantly stronger than that of NP specimens and NSCC specimens. There was significant difference in staining intensity between NIP and NP (蠂 ~ 2 = 16.847, P < 0.01 / nip and NSCC, P ~ 2 = 4.648, P < 0.05). In NIP specimens, most of the specimens were stained in the epithelial cells above the basal layer, and the basal cells were negative, but in 3 cases of malignant transformation, the staining of the basal layer of the specimens was positive. There was significant difference in the staining rate of basal layer between malignant NIP specimens and non-malignant NIP specimens (蠂 ~ 2 = 11.394; P 0.05). Conclusion SCCA is strongly positive in NIP samples, which indicates that SCCA may play a role in the occurrence and development of NIP. Clinically, the detection of SCCA can be used as a useful biological index for the diagnosis, treatment and postoperative follow-up of NIP. SCCA staining of basal cells in nip malignant transformation cases is positive, and SCCA basal staining can be considered as an index to judge the malignant transformation tendency of NIP.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R739.62
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