CCR7基因修饰的未成熟树突状细胞在大鼠高危角膜移植免疫排斥反应中的作用
发布时间：2019-07-10 20:38
【摘要】：目的研究趋化因子受体7(chemokine receptor 7,CCR7)基因重组腺病毒体外转染供体骨髓来源的未成熟树突状细胞(immature dendritic cells,imDC)对大鼠高危角膜移植免疫排斥反应的作用。方法以60只SD大鼠为受体,30只Wistar大鼠为供体,碱烧伤建立高危角膜移植模型。将SD大鼠分为空白对照组、未修饰imDC组、imDC+腺病毒空载体组(imDC+Ad组)和imDC+CCR7腺病毒组(imDCs+Ad-CCR7组),每组15只;各组受体术前7 d和术后3 d经尾静脉注入PBS液0.1 mL、供体来源的未修饰imDC 10×106个(0.1mL)、空载体腺病毒转染后的imDC 10×106个(0.1 mL)和携带CCR7腺病毒转染后的imDC 10×106个(0.1 mL);术后受体大鼠每天裂隙灯下观察角膜植片存活情况并对角膜植片的混浊、水肿及新生血管程度进行评分;术后14 d每组随机处死5只大鼠取角膜行HE切片观察。结果角膜植片存活时间:空白对照组(10.44±1.88)d,imDC组(16.00±2.18)d,imDC+Ad组(15.11±2.03)d,imDC+Ad-CCR7组(23.67±2.83)d,4组间比较差异有统计学意义(F=53.005,P=0.000);与空白对照组相比,imDC组、imDC+Ad组、imDC+Ad-CCR7组角膜植片存活时间均明显延长(均为P0.01);imDC+Ad-CCR7组较imDC组与imDC+Ad组均明显延长(均为P0.01)。角膜植片HE染色切片显示,imDC+AdCCR7组的植片轻度水肿、基质层板层纤维排列有序,未修饰imDC组和imDC+Ad组呈不同程度的水肿,基质层板层纤维排列轻度紊乱、有少量炎性细胞;空白对照组植片高度水肿、角膜基质板层纤维紊乱并出现大量炎性细胞和新生血管。结论静脉注入CCR7基因修饰的imDC可以明显延长大鼠高危角膜移植后角膜植片存活时间,抑制角膜移植免疫排斥反应。
[Abstract]:Aim to study the effect of recombinant adenoviruses containing chemokine receptor 7 (CCR7) gene into immature dendritic cells derived from donor bone marrow (immature dendritic cells,imDC) on immune rejection of high risk corneal transplantation in rats. Methods High risk corneal transplantation model was established by alkali burn in 60 SD rats and 30 Wistar rats. SD rats were divided into blank control group, unmodified imDC group, imDC adenoviral empty vector group (imDC Ad group and imDC CCR7 adenoviral group (imDCs Ad-CCR7 group) with 15 rats in each group. In each group, 0.1mL was injected into the tail vein 7 days before and 3 days after operation, unmodified imDC 10 脳 10 6 (0.1mL), imDC 10 脳 10 6 (0.1mL) and imDC 10 脳 10 6 (0.1mL) were injected into the tail vein 7 days before and 3 days after operation, the survival of corneal graft was observed under slit lamp and the opacification, edema and neovascularization degree of corneal graft were evaluated every day in the recipient group, and the unmodified imDC solution was injected into the tail vein 7 days before and 3 days after operation, and the unmodified imDC 10 脳 10 6 (0.1mL), imDC 10 脳 10 6 (imDC) and imDC 10 脳 10 6 (imDC) were injected into the tail vein every day after operation. On the 14th day after operation, 5 rats in each group were randomly killed and observed by HE section. Results the survival time of corneal graft was (10.44 卤1.88) days in blank control group, (16.00 卤2.18) days in IMDC group, (15.11 卤2.03) days in IMDC Ad group and (23.67 卤2.83) days in IMDC Ad-CCR7 group (F 鈮，
本文编号：2512877
[Abstract]:Aim to study the effect of recombinant adenoviruses containing chemokine receptor 7 (CCR7) gene into immature dendritic cells derived from donor bone marrow (immature dendritic cells,imDC) on immune rejection of high risk corneal transplantation in rats. Methods High risk corneal transplantation model was established by alkali burn in 60 SD rats and 30 Wistar rats. SD rats were divided into blank control group, unmodified imDC group, imDC adenoviral empty vector group (imDC Ad group and imDC CCR7 adenoviral group (imDCs Ad-CCR7 group) with 15 rats in each group. In each group, 0.1mL was injected into the tail vein 7 days before and 3 days after operation, unmodified imDC 10 脳 10 6 (0.1mL), imDC 10 脳 10 6 (0.1mL) and imDC 10 脳 10 6 (0.1mL) were injected into the tail vein 7 days before and 3 days after operation, the survival of corneal graft was observed under slit lamp and the opacification, edema and neovascularization degree of corneal graft were evaluated every day in the recipient group, and the unmodified imDC solution was injected into the tail vein 7 days before and 3 days after operation, and the unmodified imDC 10 脳 10 6 (0.1mL), imDC 10 脳 10 6 (imDC) and imDC 10 脳 10 6 (imDC) were injected into the tail vein every day after operation. On the 14th day after operation, 5 rats in each group were randomly killed and observed by HE section. Results the survival time of corneal graft was (10.44 卤1.88) days in blank control group, (16.00 卤2.18) days in IMDC group, (15.11 卤2.03) days in IMDC Ad group and (23.67 卤2.83) days in IMDC Ad-CCR7 group (F 鈮，
本文编号：2512877
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