HPLC法测定聚维酮碘口服液中聚维酮的含量

发布时间：2017-12-28 09:16

本文关键词：HPLC法测定聚维酮碘口服液中聚维酮的含量　出处：《中国临床药学杂志》2016年05期 　论文类型：期刊论文

【摘要】：目的建立测定聚维酮碘口服液中聚维酮含量的HPLC法。方法 HPLC法测定聚维酮的含量,色谱柱:Dikma Diamonsil汶C18柱(150 mm×4.6 mm,5μm);流动相:乙腈-水(5∶95);流速0.8 m L·min-1;紫外检测波长205 nm。结果聚维酮质量浓度在0.1~1 g·L~(-1)内与其峰面积之间具有良好线性关系,r=0.999 8。高、中、低3个浓度下方法的日内和日间精密度良好(RSD2.36%)。定量限和检测限分别为0.2、0.05 mg·L~(-1)。样品溶液在24 h内稳定(RSD=0.02%)。加样回收率为99.55%~100.80%(n=3),RSD2.05%。结论经方法学验证,本法简便、快捷、准确性好、灵敏度高,可用于测定聚维酮碘口服液中聚维酮的含量。
[Abstract]:Objective to establish a HPLC method for the determination of the content of povidone in the oral liquid of polyvidone iodine. Methods the content of povidone was determined by HPLC. The chromatographic column was Dikma Diamonsil Wen C18 column (150 mm 4.6 mm, 5 m); mobile phase: acetonitrile water (5: 95); flow rate 0.8 m L / min-1; UV detection wavelength 205 nm. Results there is a good linear relationship between the mass concentration of polyvidone in 0.1~1 g L~ (-1) and its peak area, r=0.999 8. The intra day and daytime precision of the method with 3 concentrations of high, medium and low is good (RSD2.36%). The quantitative limit and detection limit were 0.2 and 0.05 mg. L~ (-1), respectively. The sample solution is stable in 24 h (RSD=0.02%). The rate of recovery was 99.55%~100.80% (n=3) and RSD2.05%. Conclusion the method validation, this method is simple, fast, good accuracy, high sensitivity, can be used for the determination of povidone iodine in povidone oral liquid.
【作者单位】：复旦大学药学院药物分析教研室;
【分类号】：R927.2;O657.72
【正文快照】： 聚维酮碘为乙烯基吡咯烷酮均聚物与碘的复合物,它的溶液是一种消毒防腐药,能够缓慢地释放出碘而发挥抗菌作用,其特点是对组织刺激性小、性能相对稳定、毒性低,临床上常用于皮肤、黏膜的消毒[1-4]。2014年,我国农业部正式批准聚维酮碘口服液作为新药上市,用于治疗猪、鸡等牲畜

【参考文献】