蛋白激酶D1对心肌梗死大鼠心肌组织eNOS及Ang1表达的调控作用

发布时间：2018-01-14 03:26

本文关键词：蛋白激酶D1对心肌梗死大鼠心肌组织eNOS及Ang1表达的调控作用　出处：《暨南大学学报(自然科学与医学版)》2015年06期 　论文类型：期刊论文

【摘要】：目的:探讨蛋白激酶D1(PKD1)对大鼠心肌梗死后心肌组织内皮型一氧化氮合酶(e NOS)及促血管生成素1(Ang1)表达调控的影响.方法:采用冠状动脉左前降支结扎造模方法复制大鼠心肌梗死模型,术后存活达2d以上的心肌梗死大鼠随机被分为模型组、PKD1处理组与CID755673处理组,每组8只.应用逆转录聚合酶链式反应(RT-PCR)分析PKD1及其阻断剂CID755673对大鼠心肌组织中e NOS及Ang1 mRNA表达的影响,应用免疫组化法和免疫印迹法分析e NOS及Ang1的蛋白表达.结果:和心肌梗死模型组相比较,PKD1处理组大鼠心肌组织中e NOS及Ang1的mRNA和蛋白表达水平显著升高(P0.01);和PKD1处理组比较,CID755673处理组大鼠心肌组织中e NOS及Ang1的mRNA和蛋白表达水平显著下降(P0.01),接近于模型组水平.结论:PKD1显著上调大鼠心肌梗死后受损心肌组织中e NOS及Ang1的表达.
[Abstract]:Objective: To investigate the effect of protein kinase D1 (PKD1) on myocardial infarction in rats after myocardial endothelial nitric oxide synthase (E group NOS) and angiopoietin 1 (Ang1) expression. Methods: the left anterior descending coronary artery ligation model of rat model of myocardial infarction, postoperative survival of more than 2D in rats with myocardial infarction were randomly divided into model group, PKD1 treatment group and CID755673 treatment group, 8 rats in each group. The application of reverse transcriptase polymerase chain reaction (RT-PCR) analysis of PKD1 and its antagonist CID755673 on expression of E NOS and Ang1 mRNA in rat myocardium, expression of E NOS and Ang1 protein. Immunohistochemistry and Western blotting. Results: compared with model group and myocardial infarction, and protein expression levels of mRNA Ang1 and E NOS in myocardial tissue of rats in PKD1 group increased significantly (P0.01); and group PKD1 treatment, CID755673 treatment group rat heart The mRNA and protein expression levels of E NOS and Ang1 in muscle tissue were significantly decreased (P0.01), which was close to the level of model group. Conclusion: PKD1 significantly increased e NOS and Ang1 expression in damaged myocardium after myocardial infarction in rats.

【作者单位】：南阳理工学院医学实验中心;
【基金】：国家自然科学基金项目(81473438;81202791;81173372) 河南省中医内科学重点学科支撑课题项目(豫教高[2012]186号)
【分类号】：R965
【正文快照】： 蛋白激酶D1(Protein kinase D1,PKD1)是肿瘤血管形成进程中的关键调控蛋白之一[1-3],PKD1信号级联反应和胰腺癌、肝癌、胃癌、肠癌、乳腺癌、前列腺癌等的发生发展密切相关[2-7],鉴于此,抗PKD1抑制肿瘤血管的成熟而促进肿瘤细胞凋亡的治疗策略引发了学者们广泛的兴趣[5-7].PKD

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