锶盐对高浓度地塞米松作用下大鼠成骨细胞的影响

发布时间：2018-01-14 03:19

本文关键词：锶盐对高浓度地塞米松作用下大鼠成骨细胞的影响　出处：《中国骨质疏松杂志》2015年07期 　论文类型：期刊论文

【摘要】：目的探讨雷奈酸锶(Sr)对高浓度地塞米松(Dex)作用下大鼠成骨细胞(OB)的影响。方法采用大鼠骨髓基质干细胞(BMSCs)来源的成骨细胞,运用MTT试剂盒和AKP试剂盒分别测定Sr对细胞增殖和分化的影响,定量PCR方法测定Sr对高浓度地塞米松作用下成骨钙素(OC)和转化生长因子-β(TGF-β)表达的影响。结果 Sr浓度在10-5、10-4mol/L时,能够促进OB增殖和分化(P0.01);Sr浓度为10-4mmol/L能够有效拮抗高浓度Dex(10-5mol/L)对成骨细胞分化抑制作用(P0.01),并能逆转Dex对成骨相关基因OC和TGF-β的抑制作用(P0.05,P0.01)。结论 Sr能够对大鼠BMSCs来源的成骨细胞的增殖、分化有促进作用,同时10-4mol/L Sr可以拮抗Dex(10-5mol/L)对ALP和成骨相关基因的抑制作用。为Sr防治糖皮质激素性骨质疏松提供了细胞学依据。
[Abstract]:Objective to investigate the effect of strontium ralniate (Sr) on rat osteoblast (OB) induced by high concentration of dexamethasone Dex.Methods Rat bone marrow stromal cells (BMSCs) derived from osteoblasts were used. The effects of Sr on cell proliferation and differentiation were measured by MTT kit and AKP kit respectively. The effects of Sr on the expression of osteocalcin (OC) and transforming growth factor- 尾 (TGF- 尾) under high concentration of dexamethasone were determined by quantitative PCR. 10-4 mol / L could promote proliferation and differentiation of OB. Sr concentration of 10 ~ (-4) mmol 路L ~ (-1) could effectively antagonize the inhibitory effect of high concentration of Dexan10 ~ (-5) mol / L on osteoblast differentiation (P _ (0.01)). The inhibitory effect of Dex on OC and TGF- 尾 was reversed. Conclusion Sr can inhibit the proliferation of osteoblasts derived from BMSCs in rats. Differentiation can promote it. At the same time, 10-4 mol / L Sr antagonizes Dexo 10-5 mol / L). The inhibition of ALP and osteoblast-associated genes provides a cytological basis for Sr in the prevention and treatment of glucocorticoid induced osteoporosis.
【作者单位】：广东药学院附属第一医院骨外科;广东食品药品职业学院;山东省青岛即墨市人民医院肝胆外科;广东药学院生命科学与生物制药学院新药功能研究中心;
【基金】：2011年广东省医学科研基金项目(A2011317)
【分类号】：R965
【正文快照】： 4.广东药学院生命科学与生物制药学院新药功能研究中心,广州510006长期大剂量使用糖皮质激素性(glucocorticoid,GC)不仅可抑制成骨细胞(osteoblast,OB)增殖,促进其凋亡,减少骨骼中活性成骨细胞成分,导致骨形成下降,还能增加破骨细胞(osteoclast,OC)的数量和活性,促进骨吸收,造

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