表没食子儿茶素没食子酸酯联合多柔比星对肺癌A549细胞增殖与荷瘤裸鼠肿瘤生长的抑制作用

发布时间：2018-01-15 20:38

本文关键词：表没食子儿茶素没食子酸酯联合多柔比星对肺癌A549细胞增殖与荷瘤裸鼠肿瘤生长的抑制作用　出处：《中国药房》2015年10期 　论文类型：期刊论文

【摘要】：目的:研究表没食子儿茶素没食子酸酯(EGCG)联合多柔比星对肺癌A549细胞增殖与对荷瘤裸鼠肿瘤生长的抑制作用。方法:将A549细胞随机均分成模型(等容生理盐水)组、EGCG(50μmol/L)组、多柔比星(10μmol/L)组、联合用药(EGCG 50μmol/L+多柔比星10μmol/L)组。给药24、48 h后采用MTT法检测细胞活力并计算细胞增殖抑制率;采用流式细胞仪检测细胞凋亡状态并计算细胞凋亡率。皮下接种A549细胞于裸鼠背部以复制荷瘤裸鼠模型。24只模型小鼠随机均分为模型(等容生理盐水)组、EGCG(30 mg/kg)组、多柔比星(10 mg/kg)组、联合用药(EGCG 30 mg/kg+多柔比星10 mg/kg)组,复制模型成功后第3、6、9、12天时ip给药。称定小鼠肿瘤质量并计算抑瘤率;HE染色以进行肿瘤组织学观察。结果:与模型组比较,给药24、48 h后,EGCG组、多柔比星组、联合用药组细胞增殖抑制率、细胞凋亡率升高;与EGCG或多柔比星组比较,联合用药组细胞增殖抑制率、细胞凋亡率升高,差异均有统计学意义(P0.01或P0.05)。与模型组比较,EGCG组、多柔比星组、联合用药组小鼠肿瘤质量减少,抑瘤率升高,癌细胞胞核大而不规则、核质比例变小等状况有一定改善;与EGCG组或多柔比星组比较,联合用药组小鼠肿瘤质量减少,抑瘤率升高,差异均有统计学意义(P0.01)。结论:EGCG联合多柔比星能够有效抑制A549细胞的增殖和荷瘤裸鼠肿瘤的生长,其效果好于单用药。
[Abstract]:Objective: to study the EGCGs of epigallocatechin gallate. Combined with doxorubicin to inhibit the proliferation of lung cancer A549 cells and tumor growth in nude mice. Methods: A549 cells were randomly divided into model group (isovolumic saline) group. EGCG(50 渭 mol / L group, doxorubicin 10 渭 mol / L group. In combination with EGCG 50 渭 mol/L doxorubicin 10 渭 mol / L group, the cell viability was detected by MTT assay and the cell proliferation inhibition rate was calculated by MTT assay 48 h after administration of EGCG50 渭 mol/L doxorubicin (10 渭 mol / L). A549 cells were inoculated subcutaneously on the back of nude mice to replicate the tumor-bearing nude mice model. 24 model mice were randomly divided into two groups. Isovolumic saline) group. EGCG(30 mg / kg group, doxorubicin 10 mg / kg group, combined with EGCG 30 mg/kg doxorubicin 10 mg / kg group. The mice were given IP on the 12th day after the model was successfully duplicated. The tumor mass of the mice was determined and the tumor inhibition rate was calculated. Results: compared with the model group, the proliferation inhibition rate and apoptosis rate of EGCG group and doxorubicin group were increased 48 hours after administration. Compared with EGCG or doxorubicin group, the cell proliferation inhibition rate and apoptosis rate in combination group were significantly higher than those in model group (P 0.01 or P 0.05). In EGCG group, doxorubicin group, the tumor quality decreased, the tumor inhibition rate increased, the nucleus of cancer cell was large and irregular, and the ratio of nucleus to cytoplasm became smaller in combination group. Compared with EGCG group or doxorubicin group, the tumor quality decreased and tumor inhibition rate increased in combination group. Conclusion the combination of 1% EGCG and doxorubicin can effectively inhibit the proliferation of A549 cells and the growth of tumor-bearing nude mice.
【作者单位】：解放军第59中心医院药剂科;
【分类号】：R965
【正文快照】： *副主任医师。研究方向:临床药学。E-mail:2060319709@qq.com肺癌是最常见的恶性肿瘤,其恶性程度高、预后极差,特点是局部和远处转移发生早,临床作出肺癌诊断时,其中大部分患者已经是晚期[1-2]。目前,肺癌的治疗以手术为主,联合化疗为辅。然而,以细胞毒为主的化学治疗缺乏选择

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