高效液相色谱法测定驱蚊膏中避蚊胺的含量

发布时间：2018-01-16 19:39

本文关键词：高效液相色谱法测定驱蚊膏中避蚊胺的含量　出处：《药物分析杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的:应用高效液相色谱技术建立驱蚊膏中避蚊胺含量的测定方法。方法:采用Alltima C_(18)色谱柱(150 mm×4.6 mm,5μm),以甲醇-水(65∶35)为流动相,流速0.8 m L·min~(-1),检测波长210 nm。结果:在本方法中,避蚊胺的线性关系良好,线性相关系数(r)为0.999 9;精密度和稳定性良好,RSD分别为0.05%和0.18%;平均加样回收率为99.6%,RSD为1.3%。3批驱蚊膏中避蚊胺的含量测定结果分别为34.47%、34.33%、34.15%。结论:该方法经方法学验证,可作为驱蚊膏中避蚊胺的含量测定方法。
[Abstract]:Objective: to establish a HPLC method for the determination of DEE in repellent cream. Methods: the column of Alltima Cascon 18 was used to determine the content of DEE in repellent cream (150mm 脳 4.6mm). 5 渭 m, methanol-water 65: 35) as mobile phase, flow rate 0.8 mL 路min ~ (-1), detection wavelength 210 nm. Results: in this method. The linear correlation coefficient was 0.999 9; The RSD of precision and stability were 0.05% and 0.18, respectively. The average recovery was 99.6% RSD was 1.3.3 batches of mosquito repellent cream and the results were 34.47% and 34.33%, respectively. Conclusion: this method can be used as a method for the determination of DEX in mosquito repellent ointment.
【作者单位】：北京医院药学部;
【分类号】：R927.2
【正文快照】： 避蚊胺(DEET)化学名称为N,N-二乙基间甲苯甲酰胺(化学结构见图1),对多种昆虫有驱避作用,是一种高效、广谱驱避剂,对蚊、蠓、蚋有良好的驱避效果,对人畜低毒,对皮肤无刺激,无变态反应,已被广泛应用[1-2]。1957年在美国注册,是国际公认的最好驱避剂[3]。目前避蚊胺的分析方法主

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