深海链霉菌Streptomyces malaysiensis OUCMDZ-2167来源的细胞毒性产物

发布时间：2018-01-16 21:35

本文关键词：深海链霉菌Streptomyces malaysiensis OUCMDZ-2167来源的细胞毒性产物　出处：《有机化学》2017年03期 　论文类型：期刊论文

【摘要】：研究深海沉积物来源的链霉菌Streptomyces malaysiensis OUCMDZ-2167的活性天然产物.采用硅胶和凝胶柱色谱及高效液相色谱等手段,从链霉菌S.malaysiensis OUCMDZ-2167的发酵产物中分离纯化了1个新的呋喃衍生物1以及10个已知物2~11;采用质谱、紫外、红外、核磁共振和比旋光等方法将其结构分别鉴定为:4-羟甲基呋喃-2-甲酸甲酯(1)、呋喃-2,4-二甲酸二甲酯(2)、格尔德霉素(3)、17-O-去甲基格尔德霉素(4)、尼日利亚菌素(5)、尼日利亚菌素钠(6)、30-O-乙酰基尼日利亚菌素(7)、奥布菌素(8)、洋橄榄叶素(9)、surugapyrone A(10)和杀菌素A(11).采用细胞计数试剂盒-8(CCK-8)和噻唑蓝(MTT)法分别评价了化合物对K562和MCF-7及A549肿瘤细胞株的细胞毒活性,结果表明:化合物3、5~9对A549的半数抑制浓度(IC_(50))分别为2.56±0.30、0.96±0.03、0.96±0.04、0.81±0.05、7.11±0.90和4.09±0.70μmol·L-1,化合物5~9对K562的IC_(50)值分别1.46±0.11、1.08±0.03、0.48±0.10、4.68±0.19和9.60±0.50μmol·L-1,化合物5、8和9对MCF-7的IC_(50)值分别为0.29±0.15、4.05±0.50和4.81±0.70μmol·L-1;其中化合物5~8对K562和A549以及化合物5和8对MCF-7的细胞毒活性为首次报道.
[Abstract]:Study on Streptomyces Streptomyces malaysiensis from Deep-sea sediments. Active natural products of OUCMDZ-2167. Silica gel column chromatography and high performance liquid chromatography were used. A new furan derivative 1 and 10 known compounds 2C11 were isolated and purified from the fermentation products of S. malaysiensis OUCMDZ-2167. Their structures were identified by mass spectrometry, UV, IR, NMR and specific optical rotation. Gerd mycomycin, 17-O- demethyl#internal_person1# mycomycin, natigenin 5, Nigeria aspartate sodium, 30-O- acetylnigerin 7). Albumectin 8, peridoplatin 9). Surugapyrone (10) and germicin A11 (CCK-8) and thiazolyl blue (MTT) were used as cell count kit (-8CCK-8). The cytotoxic activity of the compounds to K562, MCF-7 and A549 tumor cell lines was evaluated by HPLC. The results showed that the IC50 of A549 was 2.56 卤0.30 卤0.96 卤0.03U 0.96 卤0.04, respectively. 0.81 卤0.05U 7.11 卤0.90 and 4.09 卤0.70 渭 mol 路L -1, respectively. The ICP-50 value of compound 5N9 on K562 was 1.46 卤0.11, respectively. 1.08 卤0.03U 0.48 卤0.10C 4.68 卤0.19 and 9.60 卤0.50 渭 mol 路L -1, compound 5. The ICs of 8 and 9 pairs of MCF-7 were 0.29 卤0.15 卤4.05 卤0.50 and 4.81 卤0.70 渭 mol 路L ~ (-1), respectively. The cytotoxic activities of compound 5G 8 to K562 and A549 and compounds 5 and 8 to MCF-7 were reported for the first time.
【作者单位】：中国海洋大学医药学院海洋药物教育部重点实验室;贵州省中国科学院天然产物化学重点实验室;浙江海洋大学食品与医药学院;
【基金】：国家自然科学基金(Nos.81561148012,41376148) 国家自然科学基金-广东及国家自然科学基金-山东联合(Nos.U1501221,U1406402)资助项目~~
【分类号】：R915
【正文快照】： 海洋链霉菌天然产物的研究始于1976年,截止到2016年6月的40年时间内,报道了547个海洋链霉菌天然产物[1],是海洋放线菌天然产物的重要来源.2010~2013年初报道的海洋放线菌新天然产物中,约60%来源于海洋链霉菌[2].从海洋链霉菌的天然产物中寻找具有抗肿瘤、抗病毒、抗菌和抗糖尿

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