异常黑胆质成熟剂对抑郁模型大鼠海马信号通路的影响

发布时间：2018-01-16 22:08

本文关键词：异常黑胆质成熟剂对抑郁模型大鼠海马信号通路的影响　出处：《新疆医科大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的：观察异常黑胆质成熟剂(ASMq)对抑郁症大鼠海马组织中cAMP-PKA, ERK信号通路的影响,探讨异常黑胆质成熟剂抗抑郁作用的信号通路机制。方法：采用慢性不可预见性温和应激(CUMS)诱导建立大鼠抑郁症模型,并用氟西汀(3.5mg/kg),异常黑胆质成熟剂高,中,低剂量(6.0、3.0、1.5g/kg)干预全程,通过Open-field实验和糖水消耗实验来评估大鼠抑郁活动状态。观察大鼠海马组织中cAMP含量,PKA、p-CREB, p-ERK1/2, ERK1/2的表达。结果：应激结束后与正常对照组相比,抑郁症模型组大鼠体重增加量降低、糖水消耗量减少(p0.01),水平穿越格数、竖立次数均明显降低(p0.01),处于抑郁状态；与抑郁症模型组相比,阳性对照组和异常黑胆质成熟剂中、高剂量组大鼠体重增加量上升、糖水消耗量升高(p0.01),水平穿越格数、竖立次数均明显增加(p0.01)；与正常对照组相比,抑郁症模型组大鼠海马cAMP含量及PKA、p-CREB表达降低,差异有显著性(p0.01,p0.05)。与抑郁症模型组相比,阳性对照组和异常黑胆质成熟剂中、高剂量组大鼠海马cAMP含量明显增加(p0.05), PKA、p-CREB表达明显升高(分别为p0.01,p0.05)。各组之间ERK1/2的表达没有显著性差异。与正常对照组相比,抑郁症模型组大鼠海马p-ERK1/2表达明显下降,差异有显著性(p0.01,p0.05)；与抑郁症模型组相比,阳性对照组、异常黑胆质成熟剂中剂量组海马组织p-ERK1/2表达量明显升高,差异有显著性(p0.01,p0.05)。结论：异常黑胆质成熟剂可能是通过调节cAMP-PKA, ERK信号通路来发挥抗抑郁作用。
[Abstract]:Objective: to observe the effect of abnormal black bile maturation (ASMQ) on cAMP-PKA, ERK signal pathway in hippocampus of depression rats. Methods: chronic unpredictable mild stress (CUMS) was used to induce the rat model of depression. It was treated with fluoxetine 3.5 mg 路kg ~ (-1) 路kg ~ (-1), high, medium and low dose of fluoxetine 3.5mg 路kg ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) 路kg ~ (-1)). The activity of depression in rats was evaluated by Open-field test and sugar water consumption test. The content of cAMP in hippocampus was determined by PKAP-CREB2, p-ERK1 / 2. The expression of ERK1/2. Results: compared with the normal control group, the weight gain, sugar water consumption and horizontal traversing number of depression model rats were decreased after stress. The number of erections decreased significantly (P 0.01) and was in a state of depression. Compared with the depression model group, in the positive control group and the abnormal black bilirubin maturation agent, the weight gain, the sugar water consumption and the horizontal crossing number of the rats in the high dose group were increased. The number of erections increased significantly (P 0.01). Compared with the normal control group, the content of cAMP and the expression of PKAP-CREB in the hippocampus of the depression model group were significantly lower than those in the normal control group (P 0.01). Compared with the depression model group, compared with the depression model group, the content of cAMP in hippocampus of the high dose group increased significantly in the positive control group and the abnormal black bile maturation agent, and the content of PKA in the hippocampus of the high dose group was significantly higher than that of the depression model group. The expression of p-CREB was significantly increased (p0.01, p0.05, respectively). There was no significant difference in the expression of ERK1/2 between the three groups, and the expression of p-CREB was significantly higher than that in the normal control group. The expression of p-ERK1 / 2 in hippocampus of depression model group was significantly lower than that of control group. Compared with the depression model group, the expression of p-ERK1 / 2 in hippocampal tissue in the medium dose group of abnormal black bile maturation was significantly higher than that in the depression model group (P 0.01). Conclusion: the abnormal melanoglycoid maturation agent may play an antidepressant role by regulating the cAMP-PKA, ERK signaling pathway.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R96

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