基于液滴微流控芯片技术的抗白念珠菌药物筛选研究

发布时间：2018-01-17 13:17

本文关键词：基于液滴微流控芯片技术的抗白念珠菌药物筛选研究　出处：《药学学报》2017年12期 　论文类型：期刊论文

【摘要】：本文发展了一种用于筛选抗白念珠菌药物的液滴微流控芯片技术,自行制备了液滴生成芯片,利用阿尔玛蓝在活细胞存在下能显示荧光信号的特性,将其作为指示剂和药物、白念珠菌一起包裹在液滴中,能快速检测候选化合物的抗真菌活性。以4种抗真菌药物两性霉素B、卡泊芬净、5-氟胞嘧啶、特比萘芬和新药艾迪康唑为研究对象,分别进行抗白念珠菌活性分析,并与常规的96孔板法进行比较。结果表明,液滴芯片方法孵育2 h即可实现抗真菌药物活性的快速分析,所测得的最小抑菌浓度值与96孔板法的一致率为100%,而且试剂消耗非常少。本文建立的液滴微流控芯片法简便快速,适用于大量抗真菌药物的快速筛选。
[Abstract]:In this paper, a droplet microfluidic chip technology was developed for screening anti-Candida albicans drugs. A droplet generation chip was prepared by ourselves, and the fluorescence signal could be displayed by Almaran in the presence of living cells. Candida albicans were encapsulated in liquid droplets as indicators and drugs to detect the antifungal activity of candidate compounds quickly. Four antifungal drugs, amphotericin B, carpofen 5-fluorocytosine, were used. Terbinafine and new drug Aidiconazole were used to analyze the activity of Candida albicans and compared with the conventional 96 well plate method. The rapid analysis of the antifungal activity can be realized by the liquid drop chip incubation for 2 hours. The minimum inhibitory concentration of the liquid drop chip method is 100% with that of the 96 hole plate method. The liquid droplet microfluidic chip method developed in this paper is simple and rapid and suitable for rapid screening of a large number of antifungal drugs.
【作者单位】：第二军医大学药学院;解放军91837部队卫生队;
【基金】：国家自然科学基金资助项目(81373376)
【分类号】：R91
【正文快照】： 白念珠菌(Candida albicans)是一种机会性致病真菌,通常存在于正常人肠道、口腔、上呼吸道和阴道等部位,也是医院真菌血症的主要发病原因[1]。目前的药物主要有以氟康唑(fluconazole)、伊曲康唑(itraconazole)等为代表的唑类、以两性霉素B(amphotericin B)为代表的多烯类、以

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