PD大鼠模型的行为学评价和黑质-纹状体通路中单胺类神经递质的含量变化

发布时间：2018-01-18 13:07

本文关键词：PD大鼠模型的行为学评价和黑质-纹状体通路中单胺类神经递质的含量变化　出处：《中国临床解剖学杂志》2015年02期 　论文类型：期刊论文

【摘要】：目的建立帕金森病(Parkinson’s disease,PD)大鼠模型,观测和评价其行为学改变,检测和分析PD大鼠模型黑质-纹状体通路中单胺类神经递质含量变化。方法采用6-羟基多巴胺立体定向注射至成年SD大鼠单侧前脑内侧束,制作偏侧PD大鼠模型,观测和评价其行为学改变(悬尾测试、旋转行为的旋转速度、启动时间和维持时间),通过高效液相色谱法测定和分析单胺类神经递质的含量在PD大鼠模型黑质-纹状体通路中的变化。结果 (1)PD大鼠出现了尾僵直、少动、运动迟缓、震颤、竖毛、咬尾或足、姿势和步态不稳等异常行为;(2)悬尾测试对于评价PD模型没有统计学意义,旋转行为的旋转速度、启动时间和维持时间的结果具有统计学意义且呈时间依赖性;(3)PD大鼠模型毁损侧的黑质与纹状体部位的多巴胺(Dopamine,DA)和五羟色胺(Serotonin,5-HT)含量均降低,DA的含量具有逐渐下降的趋势,术后第2、3、4及5周黑质部位的DA含量相对于正常对照组分别下降62.96%、82.88%、92.71%、91.50%,纹状体部位的DA含量分别下降77.47%、91.39%、96.25%、96.18%;术后第3、4及5周黑质部位的DA含量平均下降(89.03±5.36)%(第3、4及5周的DA含量无统计学差异),纹状体部位的DA含量平均下降(93.61±2.79)%(第3、4及5周的DA含量无统计学差异)。结论 (1)PD大鼠模型具有类似人类PD的运动症状,除旋转速度作为旋转行为经典的行为学指标外,启动时间和维持时间可作为PD模型旋转行为的辅助指标;(2)PD大鼠脑内毁损侧的黑质和纹状体部位的DA和5-HT含量均降低。
[Abstract]:Objective to establish a rat model of Parkinson's disease (PD) and to observe and evaluate its behavioral changes. The changes of monoamine neurotransmitters in the substantia nigra striatum pathway of PD rats were detected and analyzed. Methods 6-hydroxydopamine was injected into unilateral medial forebrain tract of adult SD rats by stereotactic injection. The unilateral PD rat model was made to observe and evaluate the behavioral changes (tail suspension test, rotation speed of rotation behavior, start-up time and maintenance time). The changes of monoamine neurotransmitters in the substantia nigra striatum pathway of PD rats were determined and analyzed by high performance liquid chromatography. Abnormal behaviors such as tremor, bristling, tail bite or foot, posture and gait instability; (2) tail suspension test had no statistical significance in evaluating PD model. The results of rotation speed, start-up time and maintenance time were statistically significant and time-dependent. The contents of dopamine dopamineine (DAA) and serotonin (5-HT) in the substantia nigra and striatum were decreased in the model rats. The content of DA in substantia nigra decreased by 62.96 ~ 82.88% in the 2nd week and 5th week after operation compared with the normal control group, and the content of DA in the substantia nigra decreased by 92.71%. The DA content in the striatum decreased 77.47% and 91.39%, 96.25% and 96.18%, respectively. The DA content in the substantia nigra decreased by 89.03 卤5.36 at the 4th and 5th weeks after operation (there was no significant difference in DA content between the 4th and 5th weeks after operation). The content of DA in striatum decreased by 93.61 卤2.79 on average (3rd). There was no significant difference in DA content between 4 weeks and 5 weeks. Conclusion the rat model of PPD has the similar symptoms of human PD, except rotation speed as the classic behavioral index of rotation behavior. The start-up time and maintenance time can be used as auxiliary indicators of the rotational behavior of PD model. The contents of DA and 5-HT in substantia nigra and striatum were decreased in PD rats.
【作者单位】：太仓市第一人民医院;苏州大学医学部博习临床解剖学研究所;
【基金】：江苏省卫生厅科技项目(YG201307)
【分类号】：R964;R-332
【正文快照】： content of DA and serotonin of the substantia nigra and the striatum in the lesioned lateral of PD rats.帕金森病(PD)是一种继阿尔茨海默病之后的第二大类慢性神经退行性疾病,其运动症状主要表现为随意运动减少、动作迟缓、静止性震颤、肌强直、体位不平衡及步态紊乱

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