免疫抑制剂对酵母聚糖诱导巨噬细胞Dectin-1、Toll样受体2及肿瘤坏死因子-α表达的影响

发布时间：2018-01-18 14:33

本文关键词：免疫抑制剂对酵母聚糖诱导巨噬细胞Dectin-1、Toll样受体2及肿瘤坏死因子-α表达的影响　出处：《中国现代医学杂志》2016年21期 　论文类型：期刊论文

【摘要】：目的研究霉酚酸酯和环孢素A对酵母聚糖(Zymosan A)诱导小鼠RAW264.7巨噬细胞模式识别受体Dectin-1、Toll样受体2(TLR2)表达及细胞因子肿瘤坏死因子-α(TNF-α)释放的影响。方法体外培养RAW264.7巨噬细胞,分别给予不同浓度的霉酚酸酯、环孢素A预处理细胞24 h,再利用100μg/ml Zymosan A单独刺激细胞,逆转录聚合酶链反应和流式细胞术检测细胞Dectin-1、TLR2 m RNA和蛋白水平的变化,酶联免疫吸附试验检测上清液中TNF-α浓度的变化。结果 Zymosan A单独作用巨噬细胞Dectin-1、TLR2 m RNA和蛋白水平明显上调,TNF-α浓度升高(P0.05)。Zymosan A作用于霉酚酸酯或环孢素A预处理24 h的巨噬细胞Dectin-1、TLR2 m RNA和蛋白水平较Zymosan A单独作用组明显下调,TNF-α分泌量减少(P0.05)。结论霉酚酸酯和环孢素A抑制巨噬细胞Dectin-1和TLR2的转录和翻译,并下调TNF-α的释放,降低机体对真菌病原体的清除能力,这可能是应用霉酚酸酯和环孢素A引起难控性真菌感染的机制之一。
[Abstract]:Objective to study the effect of mycophenolate mofetil and cyclosporine A on the pattern recognition receptor (Dectin-1) of RAW264.7 macrophage induced by Zymosan A. The expression of Toll like receptor 2 (TLR2) and the release of cytokine tumor necrosis factor- 伪 (TNF- 伪) were investigated. Methods RAW264.7 macrophages were cultured in vitro. The cells were pretreated with different concentrations of mycophenolate mofetil and cyclosporine A for 24 h and stimulated with 100 渭 g / ml Zymosan A. Reverse transcriptase polymerase chain reaction (RT-PCR) and flow cytometry (FCM) were used to detect the changes of TLR2 m RNA and protein levels in the cells. The concentration of TNF- 伪 in supernatant was detected by enzyme-linked immunosorbent assay (Elisa). Results Zymosan A alone acted on macrophage Dectin-1. The levels of TLR2 m RNA and protein were up-regulated. When the concentration of TNF- 伪 was increased, the macrophage Dectin-1 was pretreated with mycophenolate mofetil or cyclosporine for 24 h. The levels of TLR2 m RNA and protein were significantly down-regulated than those of Zymosan A alone. Conclusion mycophenolate mofetil and cyclosporine A inhibit the transcription and translation of Dectin-1 and TLR2 in macrophages, and down-regulate the release of TNF- 伪. It may be one of the mechanisms that mycophenolate mofetil and cyclosporine A can induce refractory fungal infection by reducing the ability of mycophenolate mofetil and cyclosporine A.
【作者单位】：安徽医科大学;空军总医院呼吸内科;
【分类号】：R96
【正文快照】： 免疫抑制剂被广泛应用于自身免疫病及移植抗宿主病的预防和治疗,目的在于控制免疫反应的加剧[1]。然而长期接受大剂量免疫抑制剂治疗,机体免疫功能遭到严重破坏,对外界病原菌的抵抗力减弱,极易发生难以控制的感染性疾病,尤其是侵袭性真菌感染,严重威胁免疫抑制患者的生存。巨

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