全柱成像毛细管等电聚焦电泳法测定4种生物制品等电点

发布时间：2018-01-20 12:46

本文关键词： 全柱成像毛细管等电聚焦 WCID-CIEF 等电点多肽激素融合蛋白疫苗病毒样颗粒胰岛素重组人乳头瘤病毒　出处：《药物分析杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的:采用全柱成像毛细管等电聚焦电泳法(WCID-CIEF),测定多肽样品、胰岛素、融合蛋白疫苗和HPV病毒样颗粒的等电点。方法:采用50 mm×100μm石英涂层毛细管。聚焦方法设置:1.5 k V聚焦1 min,3 k V聚焦4 min/8 min;检测波长:280 nm。结果:多肽样品、激素样品胰岛素和融合蛋白疫苗的等电点分别为10.98、5.87和5.86;病毒样颗粒样品HPV 31L1、HPV 33L1、HPV 45L1、HPV 52L1和HPV 58L1的实测等电点分别为8.17、7.86、7.77、7.91和7.69,并且实测等电点与理论等电点基本一致。全柱成像毛细管等电聚焦法测定生物制品专属性和精密度均符合验证要求。结论:该方法可用于生物制品的等电点分析。
[Abstract]:Objective: to determine the insulin of polypeptide samples by capillary isoelectric focusing electrophoresis with full column imaging. Isoelectric point of fusion protein vaccine and HPV virus-like particles. Methods: 50 mm 脳 100 渭 m quartz coated capillary was used. 3kV focusing 4 min/8; Results: the isoelectric point of insulin and fusion protein vaccine of peptide sample, hormone sample and fusion protein vaccine were 10.98 and 5.86, respectively. The measured isoelectric points of HPV 31L 1 HPV33L 1 HPV45L 1 HPV52L1 and HPV 58L1 were 8.17 ~ 7.86, respectively. 7.77-7.91 and 7.69. The measured isoelectric point is basically consistent with the theoretical isoelectric point. The specificity and precision of the whole column imaging capillary isoelectric focusing method for the determination of biological products meet the requirements of verification. This method can be used for isoelectric point analysis of biological products.
【作者单位】：上海博唯生物科技有限公司;
【分类号】：R927.2
【正文快照】： *第一作者Tel:(021)68036087;E-mail:zhouchaoming@bovax.com.cn全柱成像毛细管等电聚焦电泳(whole column imaging detection-capillary isoelectric focusing,WCID-CIEF)是1992年发展起来的一项检测技术,具有分析速度快,重复性好,测定准确等特点,是生物制药分析强有力的工具

【相似文献】