基质硬度对2D和3D培养乳腺癌细胞摄取纳米制剂行为的影响

发布时间：2018-01-21 05:02

  本文关键词： 基质硬度 二维/三维细胞培养 细胞形态 细胞摄取 聚苯乙烯纳米球　出处：《药学学报》2017年08期 　论文类型：期刊论文

【摘要】：以聚苯乙烯纳米球为研究对象,研究基质硬度对二维(2D)和三维(3D)培养的乳腺癌细胞摄取行为的影响。通过光催化聚合反应法制备硬度可调的聚丙烯酰胺凝胶,并在凝胶表面共价修饰胶原以支持细胞黏附,构建2D细胞培养模型;选择Ⅰ型鼠尾胶原构建3D细胞培养模型;分别接种人乳腺癌细胞MCF-7于聚丙烯酰胺凝胶表面或混悬于胶原凝胶中进行3D细胞培养;20 nm和50 nm聚苯乙烯纳米球悬液与2D或3D培养的细胞共孵育,采用激光共聚焦显微镜对细胞形态和纳米球摄取效率进行定性和定量考察。本研究发现在2D和3D培养条件下,基质硬度均参与调控肿瘤细胞肌动蛋白骨架的重构并间接影响不同粒径大小的纳米制剂的摄取行为。
[Abstract]:The polystyrene nanospheres were used as the research object. To study the effect of substrate hardness on the uptake behavior of breast cancer cells cultured with 2D and 3D), a polyacrylamide gel with adjustable hardness was prepared by photocatalytic polymerization. The collagen was covalently modified on the gel surface to adhere to the Sertoli cells, and the 2D cell culture model was constructed. The model of 3D cell culture was established by selecting collagen of type 鈪，

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