碳点的合成及天然小分子药物的光谱分析研究

发布时间：2018-01-21 05:04

  本文关键词： 上转换荧光 碳点 共振光散射技术 盐酸巴马汀 水热法　出处：《西南大学》2014年硕士论文　论文类型：学位论文

【摘要】：碳点作为一种新型的荧光碳纳米材料,拥有特殊的荧光性能,如激发和发射波长可调、光稳定性好、无光闪烁现象等,其独特的发光性质和生物相容性在生化分析、细胞成像及药物检测等领域具有较好的应用潜力。共振光散射分析方法因其操作简单、灵敏度高等优点而广泛应用于生命科学、药物分析等领域。本文从建立天然小分子药物的分析方法出发,建立了一种高灵敏检测盐酸巴马汀的方法,此外探索出了一种新的低温一步法制备高荧光量子产率碳点的方法,并将其应用在天然小分子药物检测中。具体内容包括以下几方面： (1)盐酸巴马汀与桑色素相互作用的共振光散射谱研究。在pH为4.6的HAc-NaAc缓冲液中,桑色素与盐酸巴马汀(PaＨＨ)发生化学反应,由于静电引力形成离子缔合物,导致体系在308nm处的共振光散射信号显著增强。增强的共振光散射信号与PaＨＨ的浓度在0.08-1.0μmol/L之间呈良好的线性关系,由此建立了一种测定PaＨＨ的共振光散射分析方法,检测限为8.0nmol/L。研究了反应机理,确定了最佳的反应条件,并成功用于黄藤素片及黄藤素胶囊中盐酸巴马汀含量的测定,RSD≤3.3%。 (2)一步低温水热法合成高量子产率荧光碳点。以柠檬酸为碳源,聚乙烯亚胺为钝化剂一步水热合成荧光碳点。该合成方法简便、经济、对环境友好。制备的碳点的荧光量子产率高达53%,且具有上转换荧光性质。研究了碳点的粒径、发光性质和抗干扰能力等。实验发现该碳点粒径为4nm左右,具有良好的水溶性、光稳定性和生物相容性。研究发现该碳点对pH较敏感,可用于pH传感。 (3)研究了碳点与桑色素相互作用的荧光光谱。在pH4.0HAc-NaAc环境中,表面带有大量氨基的碳点与带负电荷的桑色素由于静电引力作用,使碳点在449nm处的荧光信号和位于451nm处的上转换荧光信号发生猝灭,降低的荧光信号强度和上转换荧光信号强度与桑色素的浓度在0～300μmol/L之间呈良好的线性关系,检测限为0.6μmol/L。据此建立了双荧光定量检测桑色素的方法。讨论了体系的反应条件和外来物质的干扰,建立的方法成功用于尿样中痕量桑色素的测定,RSD≤4.5%。
[Abstract]:As a new kind of fluorescent carbon nanomaterials, carbon dots have special fluorescent properties, such as adjustable excitation and emission wavelength, good optical stability, no light flicker and so on. Its unique luminescent properties and biocompatibility have good application potential in the fields of biochemical analysis, cell imaging and drug detection. Resonance light scattering (RLS) analysis method is easy to operate. Because of its high sensitivity, it has been widely used in life sciences, drug analysis and other fields. In this paper, a highly sensitive method for the detection of palmatine hydrochloride was established based on the establishment of a method for the analysis of natural small molecular drugs. In addition, a new low-temperature one-step method for the preparation of high fluorescence quantum yield carbon points was developed and applied to the detection of natural small molecular drugs. 1) Resonance Light scattering Spectroscopy (RLS) study of the interaction between palmatine hydrochloride and mulberry pigment. In the HAc-NaAc buffer with pH 4. 6, mulberry pigment reacts with PahH hydrochloride. An ion association complex is formed by electrostatic gravity. As a result, the resonance light scattering signal of the system at 308 nm is significantly enhanced, and the enhanced resonance light scattering signal has a good linear relationship with the concentration of PaHH in the range of 0.08-1.0 渭 mol/L. A method for the determination of PaHH by resonance light scattering (RLS) was established. The detection limit was 8.0 nmol / L. The reaction mechanism was studied and the optimum reaction conditions were determined. The method has been successfully applied to the determination of palmatine hydrochloride in Huangtenin tablets and Huangtenin capsules with RSD 鈮，

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