正常菌与可吸入矿物细颗粒作用致突变性研究

发布时间：2018-01-27 04:28

本文关键词： 可吸入矿物细颗粒(IMG) Ames试验表皮葡萄球菌大肠杆菌致突变性　出处：《岩石矿物学杂志》2017年06期 　论文类型：期刊论文

【摘要】：采用Ames试验分别对蒙脱石、石英、方解石、钠长石4种可吸入矿物细颗粒及其与大肠杆菌、表皮葡萄球菌相互作用48 h后的混悬液作为受试物进行检测,分析IMG是否具有致突变性,及其与人体正常细菌群作用后,IMG/正常菌复合体是否有致突变性毒力改变。结果显示石英以及石英/大肠杆菌复合体对TA98、TA100菌株Ames试验呈阳性反应;石英/表皮葡萄球菌复合体对TA98菌株Ames试验呈阳性反应;而蒙脱石、方解石、钠长石纯矿及其与正常菌作用后的复合体Ames试验结果均为阴性。结果表明石英具有直接致突变作用;石英与正常菌菌作用后复合体依然具有致突变性,但石英表现出的碱基置换突变作用在与表皮葡萄球菌作用后消失,其机制有待进一步研究。
[Abstract]:The fine particles of four kinds of respirable minerals such as montmorillonite quartz calcite albite and Escherichia coli were determined by Ames test. The suspension of Staphylococcus epidermidis interacted for 48 h was used as the test material to analyze whether IMG had mutagenicity or not. The results showed that quartz and quartz / Escherichia coli complex were positive for Ames test of strain TA98 T100. Quartz / Staphylococcus epidermidis complex was positive for Ames test of TA98 strain. The results of Ames test of montmorillonite calcite albite and their complexes with normal bacteria were negative. The results showed that quartz had direct mutagenicity. The complex of quartz and normal bacteria is still mutagenicity, but the base replacement mutation of quartz disappeared after the interaction with Staphylococcus epidermidis, and its mechanism needs further study.
【作者单位】：四川绵阳四四医院检验科;西南科技大学;
【基金】：国家自然科学基金重点项目(41130746);国家自然科学基金面上项目(41472046)~~
【分类号】：R994.6;X513
【正文快照】： 矿物细颗粒物和微生物个体都是大气颗粒物(PM)的重要组成部分,可吸入矿物细颗粒(IMG)是指空气动力学直径小于或等于2.5μm的矿物颗粒物。研究表明沙尘大气中的颗粒物主要为硅铝酸盐颗粒(61.59%)、方解石和石英颗粒(13.59%)(陈雁菊等,2007),董发勤等(2005)对我国北方及四川北部

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