纳米羟基磷灰石与槐糖脂负载后对宫颈癌细胞的药学活性

发布时间：2018-02-01 01:03

本文关键词： 内酯型双乙酰槐糖脂纳米羟基磷灰石搭载 Hela细胞药学活性　出处：《应用与环境生物学报》2017年03期 　论文类型：期刊论文

【摘要】：槐糖脂是酵母菌产生的一种重要的生物表面活性剂,具有抗肿瘤活性.纳米羟基磷灰石(Nano-hydroxyapatite,n HA)因具有良好的生物活性及生物相容性而被广泛应用于临床载体,与药物相负载.通过n HA与内酯型双乙酰槐糖脂(Lactonic diacetyl-sophorolipids,L-SLs)最适搭载率的确定及负载后药物缓释能力的测试、细胞毒性研究、细胞形态观察,探讨搭载后的L-SLs对人宫颈癌Hela细胞的抑制作用.结果显示:最适搭载率为1:5,且L-SLs浓度为5.0 mg/m L时负载率可以达到最大值99.47%,负载后药物缓释时间为14 h,L-SLs对人宫颈癌Hela细胞的增殖有良好的抑制作用,并且细胞形态发生了明显的变化,细胞膜鼓出,体积减小,细胞肿胀裂解成碎片、细胞解体.本研究表明,L-SLs能够抑制人宫颈癌Hela细胞的增殖,n HA搭载L-SLs后能够延长槐糖脂的释放时间.
[Abstract]:As an important biosurfactant produced by Saccharomyces cerevisiae, Sophora saccharin has anti-tumor activity. Nano-hydroxyapatite is a kind of nano-hydroxyapatite. Because of its good bioactivity and biocompatibility, HAN has been widely used in clinical vectors. And drug loading. Lactonic diacetyl-sophorolipids was obtained by using n-HA and lactone diacetyl sophora saccharide. Determination of the optimal loading rate of L-SLS, determination of drug delivery ability after loading, study of cytotoxicity and observation of cell morphology. To investigate the inhibitory effect of L-SLs on human cervical cancer Hela cells, the results showed that the optimal carrying rate was 1: 5. When the concentration of L-SLs was 5.0 mg/m / L, the loading rate could reach the maximum value of 99.47%, and the drug release time was 14 h after loading. L-SLs had a good inhibitory effect on the proliferation of human cervical cancer Hela cells, and the cell morphology changed obviously, the cell membrane bulged out, the volume decreased, the cells were swollen and cracked into fragments. This study showed that L-SLs could inhibit the proliferation of human cervical cancer Hela cells and prolong the release time of locust glycolipid.
【作者单位】：齐鲁工业大学生物工程学院;齐鲁工业大学山东省微生物工程重点实验室;
【基金】：齐鲁工业大学博士基金资助~~
【分类号】：R96
【正文快照】： CLC R965在当今世界范围内,宫颈癌是女性常见的恶性肿瘤.目前最常用的治疗宫颈癌的方法是根治性切除子宫、化疗和放疗等,但这些治疗方法对患者有较严重的副作用[1].因此,寻找一种快速、有效并且无毒的药物对宫颈癌的临床治疗具有重要意义.槐糖脂是一类由酵母菌产生的糖脂类生

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