重组人Ⅱ型TNF-α受体-抗体融合蛋白对胶原诱导性关节炎模型大鼠关节滑膜细胞NF-κB信号表达的影响

发布时间：2018-02-03 23:42

  本文关键词： rhTNFR∶Fc 胶原诱导性关节炎 NF-κB信号　出处：《中国医院药学杂志》2015年22期 　论文类型：期刊论文

【摘要】：目的:探讨重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白(rhTNFR∶Fc)对胶原诱导性关节炎(CIA)模型大鼠关节滑膜细胞NF-κB信号表达的影响。方法:将40只6周龄的雌性Wistar大鼠按照随机数表法随机分为空白组、模型组、醋酸泼尼松对照组和rhTNFR∶Fc治疗组,每组10只。制备CIA模型,以Western blotting法检测关节滑膜细胞NF-κB/P65、p-ⅠκBα表达的情况。结果:rhTNFR∶Fc治疗组和醋酸泼尼松对照组可下调CIA大鼠滑膜细胞NF-κB/P65、p-ⅠκBα的表达,与模型组比较均有显著性差异(P0.05)。结论:rhTNFR∶Fc可通过降低CIA大鼠关节滑膜细胞NF-κB/P65、p-ⅠκBα蛋白表达以达到缓解炎症的作用。
[Abstract]:Objective: to investigate the effect of recombinant human tumor necrosis factor receptor-antibody fusion protein rhTNFR: Fc on collagen-induced arthritis (CIA). Effects of NF- 魏 B signal expression in synovial cells of model rats. Methods: forty 6-week-old female Wistar rats were randomly divided into blank group according to the random number table method. Model group, prednisone acetate control group and rhTNFR:Fc treatment group, 10 rats in each group. CIA model was made. Western blotting assay was used to detect NF- 魏 B / p65 in synovial cells. Results the expression of p- 魏 B 伪 in synovial cells of CIA rats was down-regulated in the control group and the control group. Results the expression of NF- 魏 B / P65 p- 鈪，

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