橙黄决明素在正常大鼠体内的药动学

发布时间：2018-02-06 00:17

本文关键词： 橙黄决明素血药浓度药动学二室模型　出处：《中国医院药学杂志》2015年14期 　论文类型：期刊论文

【摘要】：目的:建立高效液相色谱测定大鼠血浆中橙黄决明素含量的方法,研究橙黄决明素在大鼠体内的药动学过程。方法:橙黄决明素灌胃给药7.2 mg·kg-1后于5,10,20,30 min及1,2,3,4,6,8,10,12,16,24 h断尾取血,液-液萃取法处理血浆样品,HPLC测定血浆中橙黄决明素浓度,以3P97软件拟合药动学参数。结果:血浆中橙黄决明素在0.12~7.62μg·ml-1线性良好,相关系数为0.999 5(n=5),低、中、高浓度萃取回收率均95%,日内、日间RSD均10%,符合要求。灌胃给药后,大鼠血浆中其药动学过程符合二室模型。主要药动学参数(拟合值):Cmax为3.96±0.51 mg·L-1,Tmax为16.31±2.85 min,t1/2β为5.16±0.77 h,CL为0.21±0.03 L·h-1·kg-1,AUC为7.91±1.02 mg·min-1·L-1,MRT为2.26±0.97 min。结论:本实验建立的血浆处理法及HPLC法适用于大鼠体内橙黄决明素的含量测定和药动学研究,灌胃给药后橙黄决明素吸收迅速,具有较好的应用前景。
[Abstract]:Objective: to establish a high performance liquid chromatography (HPLC) method for the determination of orange cassin in rat plasma. To study the pharmacokinetic process of orange cassin in rats. Methods: the pharmacokinetic process of orange yellow cassia in rats was studied. Methods: after oral administration of orange yellow cassia in rats (7.2mg 路kg-1), the drug was given at 5: 10, 20, 30 min, and 1, 2, 2, 3, 3, 4, 6, respectively. The blood samples were collected from the tail of 8C10, 1212, 1616, 24 h. The plasma samples were treated by liquid-liquid extraction method and HPLC was used to determine the concentration of Orange cassin in plasma. The pharmacokinetic parameters were fitted with 3P97 software. Results: the linear range of cassia orange in plasma was 0.120.62 渭 g 路ml-1, the correlation coefficient was 0.999 5 渭 g 路ml-1, the correlation coefficient was 0.999 5 渭 g 路ml-1. The recovery rate of high concentration extraction was 95%, and the average RSD was 10% during the day. The pharmacokinetic process in plasma of rats was in accordance with the two-compartment model. The main pharmacokinetic parameters (the fitting value was 3.96 卤0.51 mg 路L -1). Tmax was 16.31 卤2.85 min / t 1 / 2 尾, 5.16 卤0.77 h / L and 0.21 卤0.03 L 路h -1 路kg-1 respectively. AUC was 7.91 卤1.02 mg 路min-1 路L -1. MRT was 2.26 卤0.97 min.Conclusion: the plasma treatment method and the HPLC method established in this experiment are suitable for the determination and pharmacokinetic study of Orange cassin in rats. The absorption of cassia orange by gavage is rapid, and it has a good application prospect.
【作者单位】：湖南省人民医院药学部;
【基金】：湖南省科学技术厅科技计划项目(编号:2014TT2004)
【分类号】：R965

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