气相色谱-质谱联用技术分析大气细颗粒物对小鼠肺组织代谢轮廓的影响

发布时间：2018-02-06 01:35

本文关键词： 大气细颗粒物代谢轮廓主成分分析毒性机理　出处：《分析化学》2017年08期 　论文类型：期刊论文

【摘要】：建立了基于气相色谱-质谱联用技术(GC-MS)分析大气细颗粒物(PM_(2.5))滴注对小鼠肺组织代谢轮廓的影响的研究方法。通过分析肺组织细胞内代谢物的变化,研究不同浓度PM_(2.5)对小鼠肺组织代谢的毒性机制。鼻腔分别滴注0、7.5、20.0和37.5 g/L的PM_(2.5)悬液,提取肺组织胞内物质,预处理后进行GC-MS分析,结合主成分分析法(PCA)、偏最小二乘判别分析法(PLS-DA)进行数据解析,通过PLS-DA得分图可将不同PM_(2.5)染毒浓度下的肺组织胞内物质明显区分。运用PLS-DA载荷图及模型的变量重要性因子(VIP)值,发现了7种代谢物可作为区别不同浓度PM_(2.5)下代谢组的潜在生物标志物,分别为丙氨酸、缬氨酸、亮氨酸、鸟氨酸、延胡索酸、柠檬酸、嘌呤(p0.01)。代谢途径分析结果表明,PM_(2.5)滴注使小鼠肺组织受到氧化损伤,氧化应激反应增强,抑制了三羧酸循环(TCA循环)及嘌呤代谢。本研究为深入解析PM_(2.5)致毒机理提供了新的方法及理论依据。
[Abstract]:A gas chromatography-mass spectrometry (GC-MS) method for the analysis of fine atmospheric particulate matter was established. Methods the effect of drip on the metabolic profile of lung tissue in mice was studied. The changes of intracellular metabolites in lung tissue were analyzed. To study the toxic mechanism of different concentrations of PMS-2.5) on the metabolism of lung tissue in mice, the nasal cavity was infused with 0.7.5g / L and 37.5 g / L PMSP 2.5) suspension respectively. Extracellular substances were extracted from lung tissue and analyzed by GC-MS after pretreatment. The data were analyzed with principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). The intracellular matter of lung tissue could be distinguished obviously by PLS-DA score. The PLS-DA load map and the VIPs of the model were used. Seven metabolites were found to be potential biomarkers for differentiating different concentrations of PMS-2.5) in metabolic groups, namely alanine, valine, leucine, ornithine, fumaric acid and citric acid. The results of metabolic pathway analysis showed that the mice lung tissue was damaged by oxygen oxidation and the oxidative stress reaction was enhanced. TCA cycle and purine metabolism were inhibited. This study provides a new method and theoretical basis for the further analysis of the toxic mechanism of PMSP _ (2.5).
【作者单位】：北京工商大学食品学院;北京市食品添加剂工程技术研究中心;
【基金】：国家自然科学基金项目(No.21407006)资助~~
【分类号】：O657.63;R994.6

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