3C和3CL蛋白酶及广谱抑制剂的研究进展

发布时间：2018-02-24 19:12

  本文关键词： RNA病毒 C蛋白酶 CL蛋白酶 广谱抑制剂　出处：《国际药学研究杂志》2016年03期 　论文类型：期刊论文

【摘要】：小RNA病毒和冠状病毒属于单正链RNA病毒,其家族中的病原体易导致手足口病、心肌炎、普通感冒以及严重的呼吸道和肠道疾病。3C和3CL蛋白酶都属于半胱氨酸蛋白酶,底物结合位点高度保守且具有相似的催化机制,是催化单正链RNA病毒前体蛋白裂解的关键蛋白酶,对病毒的复制有重要作用。人体中没有与其相似的蛋白酶,是目前广谱抗单正链RNA病毒研究的重要靶点。利用3C和3CL蛋白酶结构的相同点,成功发现了具有广谱作用的蛋白酶抑制剂。本文简要概述3C和3CL蛋白酶的结构、功能和广谱抑制剂的研究进展,并简要阐释抑制剂的作用机制,对该类酶的广谱抑制剂研究和相关病毒的治疗具有指导意义。
[Abstract]:Small RNA virus and coronavirus belong to single positive strand RNA virus. The pathogens in their family are susceptible to hand, foot and mouth disease, myocarditis, common cold and severe respiratory and intestinal diseases. 3C and 3CL proteases are all cysteine proteases. Substrate binding sites are highly conserved and have similar catalytic mechanisms. They are the key proteases that catalyze the cleavage of single-stranded RNA virus precursor proteins, and play an important role in viral replication. It is an important target in the research of broad-spectrum anti-Monoplar RNA virus. By using the similarities of 3C and 3CL protease structures, a wide spectrum protease inhibitor has been successfully found. In this paper, the structures of 3C and 3CL proteases are briefly summarized. The research progress of functional and broad-spectrum inhibitors, and briefly explain the mechanism of their action, are of guiding significance for the study of broad-spectrum inhibitors of this kind of enzymes and the treatment of related viruses.
【作者单位】： 沈阳药科大学制药工程学院;军事医学科学院毒物药物研究所;
【分类号】：R978.7
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本文编号：1531382