LC-MS法快速测定尼群地平纳米结晶制剂在Beagle犬中的血药质量浓度

发布时间：2018-02-26 23:24

本文关键词： 尼群地平液质联用药动学　出处：《沈阳药科大学学报》2017年01期 　论文类型：期刊论文

【摘要】：目的建立一种液相色谱质谱联用(LC-MS)方法,用于快速测定Beagle犬血浆中尼群地平的质量浓度。方法血浆样品采用液-液萃取法提取,色谱柱为Kinetex XB-C18柱(50 mm×2.1 mm,2.6μm),流动相为乙腈-体积分数0.2%甲酸溶液(体积比为70∶30)。质谱在ESI正离子模式下以多反应监测扫描方式检测。结果尼群地平的线性范围为1~500μg·L~(-1),日内和日间RSD均小于10%,准确度(RE)为-5.7%~0.1%,提取回收率大于90%,基质效应在86.11%~103.4%内。尼群地平在Beagle犬血浆中的主要药动学参数:tmax为(2.5±0.5)h;ρmax为(139.0±4.6)μg·L~(-1);t1/2为(7.1±0.9)h;AUC(0-48)为(891.4±157.1)μg·L~(-1)·h,AUC(0-∞)为(906.9±152.1)μg·L~(-1)·h。结论该测定方法适用于尼群地平纳米结晶制剂在Beagle犬体内的药物动力学研究。
[Abstract]:Objective to establish a liquid chromatography-mass spectrometry (LC-MS) method for the rapid determination of nitrendipine in plasma of Beagle dogs. The mobile phase was acetonitrile-volume fraction 0.2% formic acid solution (volume ratio 70: 30). Mass spectrometry was detected by multi-reaction scanning in the positive ion mode of ESI. Results the linear range of nitrendipine was 1 ~ 500 渭 g 路L ~ (-1), within a day. The recovery rate was more than 90%, the matrix effect was in the range of 86.11103.4%. The main pharmacokinetic parameters of nitrendipine in the plasma of Beagle dogs were 2.5 卤0.5 hmax and 139.0 卤4.6 渭 g 路L ~ (-1) 渭 g 路L ~ (-1) 路L ~ (-1) 路h ~ (-1), respectively. Conclusion this method can be used for the determination of nitrendipine in the plasma of Beagle dogs. Conclusion this method can be used for the determination of nitrendipine in the plasma of Beagle dogs with a mean recovery rate of 891.4 卤157.1 渭 g 路L ~ (-1) 渭 g 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 渭 g 路L ~ (-1) 渭 g 路L ~ (-1) of nitrendipine and 蟻 max of 139.0 卤4.6) 渭 g 路L ~ (-1) 路h ~ (-1) 路hh. Pharmacokinetic study of nanocrystallization of yunidipine in Beagle dogs.
【作者单位】：沈阳药科大学药学院;
【基金】：国家自然科学基金青年基金资助项目(81502993) 辽宁省博士科研启动基金资助项目(20141066) 辽宁省教育厅一般项目(L2014379) 沈阳药科大学中青年教师事业发展支持计划
【分类号】：R927.2;O657.63

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