新型浓度梯度微流控芯片的设计及药物诱导内皮细胞凋亡应用

发布时间：2018-02-27 07:07

本文关键词： 微流控芯片药物诱导内皮细胞凋亡　出处：《基因组学与应用生物学》2017年03期 　论文类型：期刊论文

【摘要】：为研究细胞对药物的定性定量反应,并且能够形成浓度梯度,本研究利用软件CAD、软件CFD以及软件Fluent设计了一种新形式的浓度梯度微流控芯片,注入芯片的流体能够形成浓度梯度,该芯片添加了有细胞捕获结构。研究对芯片的影响因素进行了优化,用红绿染料对芯片的浓度梯度特征进行了验证,探讨带有细胞捕获结构的浓度梯度微流控芯片实现药物对内皮细胞凋亡的可行性。通过芯片平台研究了葡萄糖以及土豆叶匀浆液诱导内皮细胞凋亡的情况。研究结果表明,新设计的浓度梯度芯片经过红、绿染料的验证,在出口主通道有八通道溶液形成了层流特性,能够保持较长的距离,形成了明显的稳定的浓度梯度。在诱导内皮细胞凋亡方面,当葡萄糖的浓度大于25μmol/L时,细胞出现了明显的抑制;而8个浓度的土豆叶匀浆稀释液对诱导内皮细胞凋亡没有明显的作用。本研究设计的微流控芯片能够实现药物诱导细胞,为筛选肿瘤药物提供了新的途径。
[Abstract]:In order to study the qualitative and quantitative reaction of cells to drugs and to form a concentration gradient, a new type of microfluidic chip with concentration gradient was designed by using software CAD, software CFD and software Fluent. The fluid injected into the chip can form a concentration gradient, and the chip has a cell capture structure. The influence factors of the chip are optimized, and the characteristics of the concentration gradient of the chip are verified with red and green dye. To investigate the feasibility of concentration gradient microfluidic chip with cell capture structure to realize apoptosis of endothelial cells. The effects of glucose and potato leaf homogenate on apoptosis of endothelial cells were studied by microarray platform. The newly designed concentration gradient chip has been verified by red and green dyes, and eight channels of solution formed laminar flow characteristics in the outlet main channel, which can maintain a long distance and form an obvious stable concentration gradient. In the aspect of inducing endothelial cell apoptosis, When the concentration of glucose was more than 25 渭 mol/L, the cells were inhibited obviously, but 8 concentrations of potato leaf homogenate had no obvious effect on inducing endothelial cell apoptosis. It provides a new way for screening tumor drugs.
【作者单位】：宜春学院医学院;宜春职业技术学院;
【基金】：江西省高等学校科技落地计划项目(编号:KJLD13090)资助
【分类号】：R96

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