FKN、PI3K及NF-κB对人外周血单个核细胞IL-6表达的的影响及缬沙坦的干预作用

发布时间：2018-02-28 08:09

本文关键词： 不规则趋化因子磷脂酰肌醇-激酶核因子κB IL- 缬沙坦　出处：《中国医科大学学报》2015年03期 　论文类型：期刊论文

【摘要】：目的探讨不规则趋化因子Fractalkine(FKN)、磷脂酰肌醇-3激酶(PI3K)、核因子κB(NF-κB)对外周血单个核细胞(PBMC)IL-6表达的影响及缬沙坦的干预作用,研究FKN影响IL-6表达的信号转导机制。方法利用密度梯度离心法分离健康人外周血单个核细胞,将提取的PBMC分为7组:空白对照组、FKN组、LY294002组(PI3K抑制剂)、PDTC组(NF-κB抑制剂)、FKN+缬沙坦组、FKN+LY294002组、FKN+PDTC组,后二组在FKN诱导PBMC细胞前分别由LY294002、PDTC预处理。各组PBMC分别在12 h和24 h后,用酶联免疫吸附实验(ELISA)法检测各组细胞培养液中的IL-6表达。结果培养12 h后,与对照组比较,FKN组IL-6明显降低(P0.05),LY294002组和PDTC组无明显差异(P0.05);与FKN组比较,FKN+缬沙坦组IL-6明显降低(P0.05),FKN+LY294002组IL-6明显升高(P0.05)、FKN+PDTC组IL-6明显降低(P0.05)。培养24 h后,各组IL-6表达无明显差异(P0.05)。结论 FKN对人外周血单个核细胞IL-6的表达具有双向调节作用,通过PI3K途径抑制IL-6的表达,而通过NF-κB途径促进IL-6的表达,总体上FKN可以抑制IL-6的表达。缬沙坦可增加FKN抑制IL-6表达的作用。
[Abstract]:Objective to investigate the effects of irregular chemokine Fractalkinesia (FKN), phosphatidylinositol 3 kinase (PI3KN) and nuclear factor- 魏 B (NF- 魏 B) on the expression of IL-6 in peripheral blood mononuclear cells (PBMCs) and the effect of valsartan on the expression of IL-6 in peripheral blood mononuclear cells (PBMCs). To study the signal transduction mechanism of FKN affecting the expression of IL-6. Methods the peripheral blood mononuclear cells (PBMC) of healthy people were isolated by density gradient centrifugation. The extracted PBMC were divided into seven groups: the control group was treated with LY294002 PI3K inhibitor PI3K and the FKN PDTC group with FKN LY294002, the latter two groups were pretreated with LY294002PDTC before FKN induced PBMC cells. PBMC was pretreated at 12 h and 24 h, respectively. Enzyme linked immunosorbent assay (Elisa) was used to detect the expression of IL-6 in the culture medium of each group. Compared with the control group, the IL-6 of the FKN group was significantly lower than that of the control group. There was no significant difference between the PDTC group and the P0.05 group, and the IL-6 of the FKN valsartan group was significantly lower than that of the control group, and the IL-6 of the FKN LY294002 group was significantly higher than that of the control group, and the IL-6 of the P0.05 FKN PDTC group was significantly lower than that of the FKN group, and the IL-6 of the FKN PDTC group was significantly lower than that of the FKN group. Conclusion FKN can regulate the expression of IL-6 in human peripheral blood mononuclear cells and inhibit the expression of IL-6 through PI3K pathway, but promote the expression of IL-6 through NF- 魏 B pathway. In general, FKN inhibited the expression of IL-6, and valsartan increased the inhibitory effect of FKN on IL-6 expression.
【作者单位】：中国医科大学附属第四医院心血管内科;
【基金】：辽宁省科技厅资助项目(2011404013-6)
【分类号】：R96

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