含低相对分子质量壳聚糖凝胶对大鼠放射性皮炎的防护作用

发布时间：2018-03-02 21:32

本文选题：放射性皮炎　切入点：高相对分子质量壳聚糖　出处：《中国医院药学杂志》2015年07期 　论文类型：期刊论文

【摘要】：目的:观察含低相对分子质量壳聚糖(相对分子质量1 000)凝胶对大鼠放射性皮炎的防治作用,并初步探讨其作用机制,为研制抗放射性皮炎新制剂提供试验依据。方法:一次性60 Co辐照60Gy造大鼠放射性皮炎模型,照射部位分别涂抹给予生理盐水(CON)、单一高相对分子质量壳聚糖凝胶(KH)、单一低相对分子质量壳寡糖(M1 000)(KL)、含相对分子质量低于1 000的壳寡糖复配高相对分子质量壳聚糖(KH+L)、比亚芬(BYF);观察不同给药组对放射性皮炎模型大鼠的保护作用。并通过皮肤含水量测定、足肿胀测定,探讨不同凝胶抗放射性皮炎的作用机制。结果:CON组、KH组、KL组及BYF组的皮炎发生率分别为100%,50%,50%,50%,皮炎评分分别为2.65,1.25,1.38,1.25;KH+L组皮炎发生率为12.5%,皮炎评分为0.125,与生理盐水组对比差异有极其显著(P0.01),与其他各组相比差异有显著性(P0.05);涂药后2 h,CON组、KH组、KL组、BYF组、KH+L组的皮肤水分含量分别为19.8%,29.2%,22.3%,25.3%,30.3%;蛋清致炎,60 min后小鼠足肿胀度分别为CON组45.5%、KH组38.9%、KL组19.5%、BYF组38.9%、KH+L组12.2%。结论:单一高相对分子质量壳聚糖凝胶、单一低相对分子质量壳聚糖凝胶、比亚芬、含低相对分子质量复配壳聚糖凝胶,均具有抗放射性皮炎作用,其中复配壳聚糖凝胶效果显著,可能与其具有保湿、抗炎作用机制有关。
[Abstract]:Objective: to observe the preventive and therapeutic effects of chitosan gel containing low molecular weight on radiation dermatitis in rats, and to explore its mechanism. Methods: the model of radiation dermatitis in rats was established by 60 Gy irradiation of 60 Gy at one time. The irradiated parts were smeared with normal saline, single high molecular weight chitosan gel, single low molecular weight chitosan oligosaccharide M1000KL, containing less than 1 000 relative molecular weight chitosan and high molecular weight chitosan chitosan. To observe the protective effect of different administration groups on rats with radiation dermatitis. Foot swelling, Results the incidence of dermatitis in the KL group and the BYF group was 100% and 50%, respectively. The dermatitis score of the KH group was 12.5 and the dermatitis score of the KH group was 0.125, which was higher than that of the normal saline group. The difference was extremely significant (P 0.01) and significant difference compared with other groups (P 0.05); the skin water content of KL group was 19.80.29. 2 min after application, the skin water content of KH group was 19.80.29. 22.30.30.The swelling degree of mouse foot after 60 min of egg white was 45.5m KH and 38.9m KL, respectively, which was the same as that of CON group (45.5m KH group), which was higher than that of KH group (38.9g / kg) after 60 min of yolk white injection, and the content of skin water in KH group was 19.80.29. 2 and 25.33.The swelling degree of mouse foot after 60 min was that of CON group. Conclusion: single, high molecular weight chitosan gel, KH L, KH L, BYF group 38.9%, KH L group 12.2.Conclusion: single high molecular weight chitosan gel, Single low molecular weight chitosan gel, Bifen, low molecular weight complex chitosan gel all have anti-radiation dermatitis effect, among which the compound chitosan gel has remarkable effect, and may have moisturizing effect with it. The mechanism of anti-inflammatory effect is related.
【作者单位】：武汉市第三医院;武汉大学药学院;武汉华纳联合药业有限公司;
【基金】：武汉市卫生和计划生育委员会科研资助项目(编号:WX12B06)
【分类号】：R965

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