牛溶菌肽线性衍生物Bac2a的生物特性及其稳定性研究

发布时间：2018-03-04 12:20

  本文选题：Baca肽　切入点：抑菌活性　出处：《生物医学工程学杂志》2017年04期 　论文类型：期刊论文

【摘要】：本研究以牛中性粒细胞牛溶菌肽的线性衍生物Bac2a为研究对象,以蜂毒素为对照肽,探讨Bac2a的生物特性及其稳定性。采用圆二色谱法、微量稀释法、噻唑蓝(MTT)法等方法测定了Bac2a的二级结构、抑菌活性、溶血活性、细胞毒性及其稳定性。研究结果表明:Bac2a在水溶液中呈无规则卷曲状,在疏水环境中则呈α-螺旋结构;Bac2a的抑菌作用较强,最小抑菌浓度(MIC)为2~32μmol/L;当肽浓度为64μmol/L时,其溶血率较低,仅为14.81%;细胞毒性小,治疗指数为3.26,细胞选择性较强;100℃处理1 h后,Bac2a依然保持较强的抑菌活性,热稳定性好。因此,Bac2a在食品、医药和其它领域都具有良好的应用前景,有望成为抗生素替代品。
[Abstract]:In this study, the biological characteristics and stability of Bac2a, a linear derivative of bovine neutrophil bovine bacteriolytic peptide, and melittin as control peptide, were studied by circular dichroism chromatography and microdilution method. The secondary structure, bacteriostatic activity, hemolytic activity, cytotoxicity and stability of Bac2a were determined by thiazolyl blue method. In the hydrophobic environment, the bacteriostatic effect of Bac2a was stronger, the minimal inhibitory concentration was 2N 32 渭 mol / L, the hemolysis rate was lower when the concentration of peptide was 64 渭 mol/L, and the cytotoxicity was small. The treatment index was 3.26, and the cell selectivity was higher than 100 鈩，

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