利奈唑胺与磷霉素对甲氧西林耐药金黄色葡萄球菌体外联合药敏的研究

发布时间：2018-03-06 16:49

本文选题：利奈唑胺　切入点：磷霉素　出处：《中国临床药理学杂志》2015年18期 　论文类型：期刊论文

【摘要】：目的评价利奈唑胺和磷霉素联用对临床分离60株甲氧西林耐药的金黄色葡萄球菌(MRSA)的体外抗菌活性。方法用棋盘法设计,微量肉汤稀释法测定不同浓度组合的抗菌药物对60株临床分离MRSA最低抑菌浓度,并计算部分抑菌浓度(FIC)指数判定联合效应。测定利奈唑胺与磷霉素联用或单用对2株MRSA临床分离菌的体外杀菌曲线。结果利奈唑胺与磷霉素联合用药后对60株MRSA临床分菌株的MIC50、MIC90较单药组降低。利奈唑胺与磷霉素联合应用对60株MRSA以协同作用为主(71.67%)。联合用药的杀菌曲线的细菌数量较单药相比降低2 log10CFU·m L-1,显示协同效应。结论利奈唑胺与磷霉素联合用药在体外对MRSA以协同作用为主,杀菌曲线也显示联合用药有协同效应。
[Abstract]:Objective to evaluate the in vitro antibacterial activity of linazolamide combined with fosfomycin against 60 strains of methicillin-resistant Staphylococcus aureus (MRSA). The minimal inhibitory concentration of antimicrobial agents with different concentrations on 60 clinical isolates of MRSA was determined by broth dilution method. The in vitro bactericidal curves of two clinical isolates of MRSA with or without linazolamide and fosfomycin were determined. Results 60 strains of MRSA were treated with linazolamide and fosfomycin after the combination of linazolamide and fosfomycin. The MIC50 MIC90 of clinical substrains was lower than that of single drug group. The synergistic effect of linazolamide combined with fosfomycin on 60 strains of MRSA was 71.67%. The number of bacteria in the bactericidal curve of the combined drug was 2 log10CFU 路ml -1 lower than that of the single drug, which showed synergistic effect. The synergistic effect of the combination of linazolamide and fosfomycin on MRSA in vitro was discussed. The bactericidal curve also showed that the combined drug had synergistic effect.
【作者单位】：解放军总医院药物临床研究中心;
【基金】：“十二五”国家重大科技专项基金资助项目(2012ZX09303004-002) 国家自然科学基金青年基金资助项目(30370615)
【分类号】：R96

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