阳离子交换-高效液相色谱-电化学法测定人血浆中儿茶酚胺的浓度

发布时间：2018-03-06 17:11

  本文选题：儿茶酚胺　切入点：阳离子交换　出处：《中国医院药学杂志》2015年14期 　论文类型：期刊论文

【摘要】：目的:采用弱阳离子固相萃取小柱(WCX-SPE)优化人血浆儿茶酚胺检测实验条件,以减少血浆用量,提高实验稳定性,建立操作简单、可靠的人血浆儿茶酚胺浓度检测方法。方法:采用waters WCX固相萃取小柱提取血浆中儿茶酚胺,采用高效液相色谱-电化学检测法测定儿茶酚胺,并对其线性范围、方法回收率、提取回收率、日间日内精密度及稳定性进行考察。结果:去甲肾上腺素(NE)在50~2 000 pg·ml-1(n=6,r=0.998 5),肾上腺素(E)在50~2 000 pg·ml-1(n=6,r=0.999 3)浓度范围内线性关系良好,最低检测限分别为25,50 pg·ml-1(S/N≥3),NE、E样品的平均提取回收率分别为89.6%、91.5%,平均方法回收率分别为100.6%,99.4%,平均日间RSD分别为5.1%,4.3%,平均日内RSD分别为3.9%,3.6%。结论:采用WCX固相萃取小柱提取血浆中儿茶酚胺灵敏度高,操作简便,结果准确可靠,适合于临床诊疗需要。
[Abstract]:Objective: to optimize the detection conditions of human plasma catecholamine by using weak cationic solid phase extraction column (WCX-SPE), in order to reduce the amount of plasma, improve the stability of the experiment, and establish a simple operation for the determination of catecholamine in human plasma. A reliable method for the determination of catecholamine in human plasma. Methods: the catecholamine in plasma was extracted by waters WCX solid-phase extraction column, and determined by high performance liquid chromatography-electrochemical detection. The recovery rate, intra-day precision and stability were investigated. Results: the linear relationship was good in the concentration range of 50 ~ (2 000) PG 路ml ~ (-1) n ~ (-1) n ~ (-1) n ~ (-1) N ~ (-1) N ~ (-1) N ~ (-1) N ~ (-1) N ~ (-1) N ~ (-1) N ~ (-1) N ~ (-1) N ~ (-1) N ~ (-1) N ~ (-1) N ~ (-1) N ~ (-1) N ~ (-1) N ~. The minimum detection limit was 255pg 路ml-1(S/N 鈮，

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