HPLC法同时测定速克感冒胶囊中5种成分的含量

发布时间：2018-03-08 05:37

本文选题：中西药复方制剂　切入点：忍冬藤　出处：《药物分析杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的:建立高效液相色谱法同时测定速克感冒胶囊中绿原酸、马来酸氯苯那敏、乙酰水杨酸、蒙花苷和次野鸢尾黄素。方法:采用Venusil XBP C_(18)色谱柱(4.6 mm×250 mm,5μm),以乙腈(A)-0.5%磷酸溶液(B)为流动相,梯度洗脱,流速1.0 mL·min~(-1),检测波长327 nm(绿原酸)、260 nm(氯苯那敏)、276 nm(乙酰水杨酸)、334 nm(蒙花苷)和266 nm(次野鸢尾黄素)。结果:5种待测成分分离度良好,阴性无干扰;绿原酸、马来酸氯苯那敏、乙酰水杨酸、蒙花苷和次野鸢尾黄素进样量分别在0.078~0.388μg(r=0.999 9)、0.196~0.978μg(r=0.999 7)、4.066~20.332μg(r=0.999 9)、0.165~0.825μg(r=0.999 9)和0.048~0.238μg(r=0.999 0)范围内线性关系良好;平均回收率分别为96.8%、101.2%、96.8%、100.0%和101.3%,RSD分别为1.8%、2.4%、1.1%、1.9%和3.9%。3批样品中绿原酸、马来酸氯苯那敏、乙酰水杨酸、蒙花苷和次野鸢尾黄素的平均含量分别为0.88~1.00、2.05~2.20、110.02~116.00、2.18~2.45和0.06~0.10 mg·粒~(-1)。结论:该方法可作为评价和监控速克感冒胶囊质量的方法。
[Abstract]:Objective: to establish a HPLC method for the simultaneous determination of Lv Yuan, chlorphenamine maleate and acetylsalicylic acid in Sukegan capsules. Methods: the chromatographic column of Venusil XBP C _ (18) was used as a column of 4.6 mm 脳 250 mm ~ (5 渭 m) with acetonitrile (An) -0.5% phosphoric acid solution as mobile phase and gradient elution. The flow rate was 1.0 mL 路min ~ (-1) 路min ~ (-1) 路min ~ (-1). The detection wavelength was 327 nm (chlorphenicylate) 260 nm (chlorphenicylate) 276 nm (anthocyanin) and 266 nm (irisin). Results the separation of five components to be determined was good, negative and undisturbed, Lv Yuan acid, chlorphenamine maleate, and chlorphenamine maleate, respectively. The linear range of acetylsalicylic acid, anthocyanin and iris lutein was 0.078 ~ 0.388 渭 g / g ~ 0.999 9 ~ 0.196 0.196 ~ 0.978 渭 g / r ~ 0.999 ~ 7 ~ 4.066 ~ 20.332 渭 g / g ~ 0.999 ~ (9) 渭 g ~ 0.1650.825 渭 g / r 0.999 ~ 9) and 0.0480.238 渭 g / r ~ 0.999 ~ 0), respectively. The average recoveries were 96.81.296.8100.0% and 101.33%, respectively, and the RSD was 1.81.19% and 3.9.3%, respectively, and the RSD was 1.81.90% and 3.9.3% respectively in the samples of chloric acid and chloric acid, and the average recoveries were 96.81.296.8100.0% and 101.33%, respectively, in the range of 0.1650.825 渭 g / r 0.999 9) and the average recoveries were 96.81.296.8100.0% and 101.33%, respectively. The average contents of acetylsalicylic acid, menthoside and Iris were 0.88, 1.00, 2.05, 2.20, 110.02, 116.00, 2.180.45 and 0.06, 0.10 mg 路L ~ (-1), respectively. Conclusion: this method can be used to evaluate and monitor the quality of Suoke Ganmao capsules.
【作者单位】：河南省食品药品检验所;河南省中医药研究院;河南中医药大学;
【基金】：2015年度国家药品标准提高工作(中药)科研专项(WX-15-25)
【分类号】：O657.72;R927.2

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