新型香豆素类氟离子荧光探针的合成及细胞成像研究

发布时间：2018-03-10 16:25

本文选题：香豆素　切入点：氟离子　出处：《化学学报》2017年04期 　论文类型：期刊论文

【摘要】：设计合成了一类基于分子内电荷转移(Intramolecular Charge Transfer,ICT)的香豆素类F~-荧光探针CS1,CS2和CS3,经~1H NMR,~(13)C NMR,IR和HRMS表征了相应探针的结构,并解析了探针CS3的晶体结构.通过核磁和质谱实验验证了探针与F~-的作用机理是氟化物脱硅基.光谱分析实验结果显示,CS1,CS2和CS3均具有较好的选择性和灵敏度,且均能成功实现人乳腺癌细胞(MCF-7)中F~-的检测.
[Abstract]:A class of coumarin FG-fluorescence probes CS1C / CS2 and CS3 were designed and synthesized based on intramolecular charge transfer molecular Charge transfer (ICTT). The structures of the probes were characterized by 1H NMR-13C-NMR-IR and HRMS. The crystal structure of the probe CS3 was analyzed. The mechanism of the interaction between the probe and F- was verified by NMR and MS. The results of spectroscopic analysis showed that both CS2 and CS3 had better selectivity and sensitivity. The detection of FN-in human breast cancer cell line MCF-7 can be successfully achieved.
【作者单位】：西北大学化学与材料科学学院合成与天然功能分子化学教育部重点实验室;
【基金】：国家自然科学基金(Nos.21472148,21072158) 陕西省留学人员科技活动择优资助项目(No.20151190) 西北大学优秀青年学术骨干支持计划资助~~
【分类号】：R914.5;O657.3

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