HPLC法测定头孢克洛原料中杂质C的研究

发布时间：2018-03-13 05:33

  本文选题：高效液相色谱法　切入点：头孢克洛　出处：《中国抗生素杂志》2015年08期 　论文类型：期刊论文

【摘要】：目的建立头孢克洛原料中杂质C(D-头孢克洛)的HPLC测定方法。方法采用C18(4.6mm×250mm,5μm)色谱柱;流动相为0.78%磷酸二氢钠溶液(取磷酸二氢钠7.8g,加水溶解并稀释至1000m L,用磷酸调p H值至4.0)-乙腈(90:10);流速1.0m L/min;检测波长254nm;柱温35℃。结果杂质C在1.22~2.85μg范围内线性关系良好(r=0.9995,n=5),平均回收率为99.89%,RSD为0.59%(n=9),定量限为2.038ng。结论该方法简便、准确、重现性好,可用于头孢克洛原料中杂质C的检测。
[Abstract]:Objective to establish a HPLC method for the determination of cefloxacin impurity in cefaclor. Methods the chromatographic column of C18C 4.6mm 脳 250mm ~ 5 渭 m was used. The mobile phase consisted of 0.78% sodium dihydrogen phosphate solution (sodium dihydrogen phosphate 7.8 g, dissolved in water and diluted to 1000 mL), adjusted pH value with phosphoric acid to 4.0% -acetonitrile 90: 10 min, flow rate 1.0 mL / min, detection wavelength 254 nm, column temperature 35 鈩，

本文编号：1605062