基于蛋白质组学的药物代谢酶与转运体定量分析研究进展

发布时间：2018-03-13 18:00

本文选题：蛋白质组学　切入点：质谱技术　出处：《药学学报》2015年06期 　论文类型：期刊论文

【摘要】：随着药物开发过程的不断完善和研究技术的进步,人们对药物在体内的代谢过程和代谢机制有了更深层次的认识。药物在体内的代谢由药物代谢酶和转运体共同介导,因此,对药物代谢酶和转运体的研究已经成为药物研发过程中的重要内容。传统免疫测定法不能真实反映体内药物代谢酶和转运体的表达水平,且存在灵敏度低、特异性差的缺点。本文主要从蛋白质组学的角度出发,结合近年来生物质谱技术的发展,对药物代谢酶和转运体的定量研究做一综述介绍。
[Abstract]:With the continuous improvement of drug development process and the progress of research technology, people have a deeper understanding of the metabolic process and mechanism of drugs in vivo. Drug metabolism in vivo is mediated by drug metabolizing enzymes and transporters. The study of drug metabolizing enzymes and transporters has become an important part of drug development. Traditional immunoassay can not truly reflect the expression level of drug metabolizing enzymes and transporters in vivo and has low sensitivity. In this paper, from the perspective of proteomics, combined with the development of bio-mass spectrometry in recent years, the quantitative study of drug metabolizing enzymes and transporters is reviewed.
【作者单位】：吉林大学生命科学学院药物代谢研究中心;
【基金】：国家自然科学基金资助项目(21105036,81373374,81430087)
【分类号】：R96

【共引文献】