猪源纤维蛋白贴对大鼠肝脏及肝素化兔臀大肌创面的止血作用

发布时间：2018-03-13 18:06

本文选题：纤维蛋白贴　切入点：肝脏出血　出处：《中国药房》2017年28期 　论文类型：期刊论文

【摘要】：目的:研究猪源纤维蛋白贴(DBT)对大鼠肝脏和肝素化兔臀大肌出血创面的止血效果。方法:48只大鼠和24只兔均随机分为假手术组、手术对照组(纱布止血)、医用胶原蛋白海绵组和DBT组,除假手术组外,其他各组动物分别建立大鼠肝脏出血模型或肝素化兔臀大肌静脉出血模型。记录各组动物止血时间、计算出血量;观察大鼠术后3、13周DBT降解、肝脏创面愈合及粘连情况;考察医用胶原蛋白海绵组和DBT组肝素化兔的再渗血率。结果:与假手术组比较,手术对照组动物的止血时间和出血量均显著升高(P0.01);与手术对照组比较,DBT组和医用胶原蛋白海绵组动物的止血时间和出血量均显著减少(P0.01)。术后3周和13周,各组大鼠创面均有不同程度粘连,但粘连评分比较差异无统计学意义(P0.05);术后13周两给药组大鼠肝脏肝缘稍钝,但基本已恢复至术前状态,愈合状况良好;DBT能降解、吸收完全。DBT组兔的再渗血率为33.3%,医用胶原蛋白海绵组为66.7%。结论:DBT对脆性脏器和凝血功能障碍机体具有较好的止血效果,且能降解吸收;其作用效果与医用胶原蛋白海绵相当,且黏合强度略强于后者。
[Abstract]:Objective: to study the hemostatic effect of porcine fibrin paste (DBT) on rat liver and heparinized rabbit gluteus maximus bleeding wound. Methods: forty eight rats and 24 rabbits were randomly divided into sham-operated group. The control group (gauze hemostasis group, medical collagen sponge group and DBT group), except sham-operation group, established rat liver hemorrhage model or heparinized rabbit gluteus maximus vein hemorrhage model, and recorded the hemostasis time of each group. To calculate the amount of blood loss, to observe the degradation of DBT, the healing and adhesion of liver wound, and to investigate the reosmosis rate of heparinized rabbits in the medical collagen sponge group and DBT group. Results: compared with the sham operation group, The hemostasis time and the amount of bleeding in the control group were significantly increased compared with the control group, the hemostasis time and the amount of bleeding in the DBT group and the medical collagen sponge group were significantly decreased compared with the control group. The hemostasis time and the amount of bleeding in the DBT group and the medical collagen sponge group were significantly decreased 3 and 13 weeks after operation. There was different degree of adhesion in the wound of each group, but there was no significant difference in the adhesion score between the two groups, the hepatic margin of the rats in the two groups was slightly blunt at 13 weeks after operation, but it had basically recovered to the state of preoperation, and the healing condition was good that DBT could be degraded. The reosmosis rate of absorbent complete. DBT group was 33. 3%, and that of medical collagen sponge group was 66. 7%. Conclusion\% DBT has a good hemostatic effect on fragile organs and clotting dysfunction, and it can be degraded and absorbed, and its effect is similar to that of medical collagen sponge. The bonding strength is slightly stronger than that of the latter.
【作者单位】：广州军区广州总医院药剂科/广州市老年慢病患者合理用药重点实验室;广东药科大学中药学院;南方医科大学药学院;南方医科大学检验与生物技术学院;广州市拜特凇医药科技有限公司;
【基金】：国家科技重大新药创制项目(No.2013ZX09J13110) 中央军委2016年度军队后勤科研计划项目(No.CGZ16C009) 广州市科技计划项目(No.201509010012、201704020173)
【分类号】：R965

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