他达拉非对大鼠睾丸缺血再灌注损伤的保护作用

发布时间：2018-03-14 14:25

本文选题：睾丸扭转　切入点：再灌注损伤　出处：《中华男科学杂志》2015年03期 　论文类型：期刊论文

【摘要】：目的:探讨5型磷酸二酯酶抑制剂他达拉非在大鼠睾丸缺血再灌注模型中的保护作用。方法:将84只健康成年雄性SD大鼠随机分成4组,A组:假手术组;B组:小剂量他达拉非组;C组:大剂量他达拉非组;D组:安慰剂组。每组21只,B、C、D组建立右侧睾丸扭转复位模型(720°,2 h)。术后立即予A、B组按他达拉非剂量为0.5 mg/(kg·d)灌胃,C组按他达拉非剂量为2 mg/(kg·d)灌胃,D组用等剂量的生理盐水灌胃,用药3、7、14 d后,取各组7只大鼠扭转侧睾丸,检测睾丸组织超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,并光镜检测睾丸组织病理学参数。结果:B组[3 d:(254.46±7.43)n U/mg prot;7 d:(278.49±8.33)n U/mg prot;14 d:(317.99±3.31)n U/mg prot]、C组[3 d:(277.12±8.80)n U/mg prot;7 d:(309.40±2.14)n U/mg prot;14 d:(320.39±4.72)n U/mg prot]SOD活性均显著高于同期D组[3 d:(223.21±4.65)n U/mg prot;7 d:(231.45±4.16)n U/mg prot;14 d:(248.28±5.74)n U/mg prot],MDA含量均显著低于同期D组。C组3、7 d的SOD活性显著高于同期B组(P0.01),而MDA含量显著低于同期B组(P0.01),14 d时的SOD与MDA两组间没有显著性差异(P0.05)。睾丸组织的病理学改变:A组睾丸组织未见明显损伤,B组3、7、14 d之间病理学参数(生精上皮层数、睾丸结构得分、生精小管直径)存在显著差异(P0.01),B组14 d时和C组7 d时测得的生精上皮层数、睾丸结构评分、生精小管直径与A组14 d时没有显著性差异。结论:他达拉非可明显改善睾丸扭转后的缺血再灌注损伤,改善程度与用药时间、用药剂量呈明显的相关性。
[Abstract]:Objective: to investigate the protective effect of Tadalafil, a type 5 phosphodiesterase inhibitor, on rat testicular ischemia-reperfusion model. Methods: 84 healthy adult male SD rats were randomly divided into 4 groups: group A: sham operation group: group B: small rats. Tadalafil group C group: large dose tadalafil group D group: placebo group. The right testicular torsion reduction model was established in each group of 21 rats. The right testicular torsion reduction model was established immediately after operation. Group D was perfused with the same dose of normal saline, and the rats in group D were given intragastric administration of normal saline at a dose of 2 mg/(kg 路d. After treatment for 3 days and 7 days, the testis of 7 rats in each group were taken out, and the activity of SOD and the content of malondialdehyde (MDA) in the testis were measured. The histopathological parameters of testis were examined by light microscope. Results the activity of SOD in group B [3: 254.46 卤7.43nU / mg prot + 7 d] was significantly higher than that in group D [317.99 卤3.31nU / prot] C [3: 277.12 卤8.80n U / mg / mg / mg / SOD] SOD activity was significantly lower than that in group D [3d: 277.12 卤8.80n U / mg / mg / mg / mg prot 14 d + 320.39 卤4.72n U / mg / mg prot] SOD activity was significantly lower than that in group D [223.21 卤4.65nU / mg proprot7d-1 卤4.16n Uprotprot1 卤4.16nU / P / SOD] in the same time period [3d: 277.12 卤8.80n U / mg / mg / mg / mg / mg / v] the activity of SOD in group C was significantly lower than that in group D [3: 277.12 卤8.80n U / mg / mg / mg / mg] of the same day group [223.21 卤4.65nU / mg / mg / mg] of the same period [3: 277.12 卤8.80n U / mg / mg / mg / SOD]. The activity of SOD in group D was significantly higher than that in group B at 3 ~ 7 days, while the content of MDA was significantly lower than that in group B at day 14. There was no significant difference between SOD and MDA at day 14. The pathological changes of testis in group A were not significantly damaged. The pathological parameters (the number of spermatogenic epithelium) were observed in group B between 3 and 7 days after injury. There were significant differences in testicular structure and seminiferous tubule diameter. The number of spermatogenic epithelium and testicular structure were measured at 14 d and 7 d in group B and C respectively. There was no significant difference in the diameter of seminiferous tubules between group A and group B.Conclusion: Tadalafil can significantly improve the ischemia-reperfusion injury after testicular torsion.
【作者单位】：温州医科大学附属第一医院泌尿外科;温州医科大学实验动物中心;
【基金】：温州市科技局课题(Y20110166)~~
【分类号】：R965

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