林肯链霉菌高产菌株选育研究

发布时间：2018-03-15 22:01

  本文选题：林肯链霉菌　切入点：三重复合诱变　出处：《中国抗生素杂志》2017年08期 　论文类型：期刊论文

【摘要】：为进一步提高林可霉素生产发酵水平,本文以林肯链霉菌LK15-35为出发菌株,采用氯化锂(Li Cl)、常压室温等离子体(ARTP)和紫外线(UV)三重复合诱变,首先用Li Cl预处理4h,然后用ARTP照射60s,再用UV照射30s,最终获得高产突变株LK16-77。结果表明,摇瓶发酵单位提高30.0%,经180吨罐生产验证,平均放罐效价提高21.4%。稳定性传代验证证明该突变株高产性能遗传稳定。
[Abstract]:In order to further improve the fermentation level of lincomycin, Streptomyces Lincoln LK15-35 was used as the starting strain. The high yield mutant LK16-77 was obtained by pretreatment with Li Cl for 4 h, then irradiated with ARTP for 60 s and then irradiated with UV for 30 s. The results showed that the fermentation unit of shaking flask was increased by 30.0% and verified by 180 ton pot production. The average titer of the mutant was increased by 21.4.The stability test proved that the mutant was genetically stable in high yield.
【作者单位】： 宁夏泰瑞制药股份有限公司;
【分类号】：R915
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本文编号：1616994