肌肽对脂多糖诱导的人皮肤微血管内皮细胞氧化应激及促炎症反应的影响

发布时间：2018-03-16 04:00

本文选题：肌肽　切入点：脂多糖　出处：《中国皮肤性病学杂志》2015年06期 　论文类型：期刊论文

【摘要】：目的探讨肌肽(carnosine)对脂多糖(lipopolysaccharide,LPS)诱导的人皮肤微血管内皮细胞(human dermal microvessel endothelial cells,HDMEC)氧化应激及促炎症反应的影响。方法建立脂多糖(1μg/m L)诱导的人皮肤微血管内皮细胞损伤模型,MTT法检测其细胞活性,荧光法测定细胞内活性氧族物质(ROS),试剂盒检测一氧化氮(NO)、8-羟基脱氧鸟苷(8-OHDG)、3-硝基酪氨酸(3-NT)、白介素(IL)-1β,IL-6、肿瘤坏死因子(TNF-α)含量,超氧化物歧化酶(SOD)及谷胱甘肽过氧化酶(GSH-Px)酶活性。结果肌肽预孵育或与脂多糖处理对人皮肤微血管内皮细胞细胞活力无明显影响,脂多糖增加胞内ROS和胞外上清液NO水平,增加胞内MDA,8-OHDG及3-NT含量,增加促炎症因子IL-1β,IL-6及TNF-α产生,降低SOD和GSH-Px酶活性,而肌肽可以恢复脂多糖所致的上述效应。结论肌肽对脂多糖所致的人皮肤微血管内皮细胞损伤具有保护作用。
[Abstract]:Objective to investigate the effects of carnosine on oxidative stress and inflammatory response induced by lipopolysaccharide (LPS) in human dermal microvessel endothelial cells (HDMECs) induced by lipopolysaccharide. Methods the injury model of human skin microvascular endothelial cells induced by lipopolysaccharide 1 渭 g / mL was established. The cell activity was detected by MTT assay. The reactive oxygen species (Ros) in cells were determined by fluorescence method, and the contents of nitric oxide 8-hydroxy-deoxyguanosine (8-OHDGN) 3-nitrotyrosine 3-NTN, interleukin-1 尾 sil 6 and tumor necrosis factor TNF- 伪 were detected by fluorescence assay. Results Carnosine preincubated or treated with lipopolysaccharide had no significant effect on the viability of human skin microvascular endothelial cells. Lipopolysaccharide increased the level of intracellular ROS and no in extracellular supernatant. Increase the contents of MDA-8-OHDG and 3-NT, increase the production of IL-1 尾 -IL-6 and TNF- 伪, and decrease the activities of SOD and GSH-Px. Conclusion Carnosine can protect human skin microvascular endothelial cells from lipopolysaccharide induced by lipopolysaccharide.
【作者单位】：十堰市太和医院皮肤科;
【分类号】：R96

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