皮下注射重组人生长激素对血管性痴呆大鼠学习记忆能力、空间探索能力的影响及其机制探讨

发布时间：2018-03-16 18:20

  本文选题：血管性痴呆　切入点：重组人生长激素　出处：《山东医药》2017年47期 　论文类型：期刊论文

【摘要】：目的观察重组人生长激素(rh GH)对血管性痴呆(VD)大鼠学习记忆能力、空间探索能力的影响,并探讨其可能机制。方法 45只大鼠随机分为实验组、模型组、正常组各15只,其中实验组和模型组通过不同时间点双侧颈总动脉结扎法(结扎左侧颈总动脉3 d后再结扎右侧颈总动脉)制备VD模型,正常组用同样方法分离双侧颈总动脉,但不结扎颈总动脉;造模后第1天开始,实验组大鼠颈部皮下注射rh GH(0.2 IU/100 g),正常组和模型组给予等量生理盐水,1次/d,连续28 d。采用水迷宫实验评价大鼠学习记忆能力及空间探索能力,ELISA法检测血清、皮质、海马中血管内皮生长因子(VEGF)、胰岛素样生长因子1(IGF-1)、生长激素(GH),TUNEL凋亡染色观察神经元凋亡情况[以积分光密度值(IOD值)表示]。结果与正常组比较,实验组第1~4天及模型组第1~6天逃避潜伏期长(P均0.05);与模型组比较,实验组第2、4、5、6天逃避潜伏期短(P均0.05)。与模型组比较,实验组、正常组经过平台次数多(P均0.05)。与模型组比较,实验组血清、皮质、海马中VEGF、IGF-1、GH水平升高(P均0.05);与正常组比较,模型组血清、皮质、海马中VEGF、IGF-1、GH水平降低(P均0.05)。与模型组比较,实验组、正常组海马TUNEL凋亡染色IOD值降低(P均0.05)。结论 rh GH可改善VD大鼠学习记忆能力、空间探索能力,其机制可能与其可增加血清、皮质、海马中VEGF和IGF-1水平有关。
[Abstract]:Objective To observe the effects of recombinant human growth hormone (RH GH) on vascular dementia (VD) learning and memory ability of rats, effects of space exploration, and to explore its possible mechanism. Methods 45 rats were randomly divided into experimental group, model group, normal group with 15 rats in each group, the experimental group and model group through different at the time of bilateral common carotid artery occlusion (ligation of the left carotid artery 3 d after ligation of right common carotid artery) preparation of VD model, the normal group with the same method, separation of bilateral carotid artery without ligation of common carotid artery; the first day after modeling, the subcutaneous injection of RH rats (GH group 0.2 IU/100 g), the normal group and model group were given normal saline, 1 times /d, for 28 d. by using water maze test to evaluate the learning and memory ability of rats and space exploration, detection of serum ELISA, cortex, vascular endothelial growth factor in hippocampus (VEGF), insulin-like growth factor 1 (IGF-1) students. 闀挎縺绱，

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