反相高效液相色谱-脉冲电化学检测法测定重组人生长激素中异丙基-β-D-硫代半乳糖苷（英文）

发布时间：2018-03-16 23:08

  本文选题：反相高效液相色谱法　切入点：脉冲电化学检测　出处：《色谱》2017年03期 　论文类型：期刊论文

【摘要】：采用反相高效液相色谱(RP-HPLC)-脉冲电化学检测(PED)法测定了重组人生长激素(rhGH)中的诱导剂异丙基-β-D-硫代半乳糖苷(IPTG)。rhGH样品经超滤离心后,用超纯水提取,经C18色谱柱分离,用脉冲电化学器检测。结果表明,样品中添加0.02~0.10 mg/L的IPTG,其回收率为100%~102%,相对标准偏差(n=3)小于10%。在优化的条件下,IPTG的检出限可达1μg/L(0.1 pmol,25μL)。该方法简便高效,具有良好的灵敏度、回收率和重复性。
[Abstract]:The inducer isopropyl- 尾 -D- thiogalactoside (IPTGG) in recombinant human growth hormone (rhGH) was determined by reversed-phase high performance liquid chromatography (RP-HPLC-) -pulsed electrochemical detection (PED) method. After ultrafiltration centrifugation, the samples were extracted with ultrapure water and separated by C18 column. The results of pulse electrochemical detector showed that the recovery rate of the IPTGs added 0.02 ~ 0.10 mg/L was 100 ~ 102and the relative standard deviation was less than 100.The detection limit of IPTG could reach 1 渭 g / L ~ 0.1pmol ~ (-1) ~ (25 渭 L) under the optimized conditions. The method was simple and efficient, and had good sensitivity. Recovery and repeatability.
【作者单位】： 复旦大学分析测试中心;上海张江生物技术有限公司;
【分类号】：R927;O657
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本文编号：1622070