HPLC法测定人血清中拉莫三嗪浓度的方法改进

发布时间：2018-03-18 07:24

  本文选题：拉莫三嗪　切入点：治疗药物监测　出处：《中国药房》2015年32期 　论文类型：期刊论文

【摘要】：目的:对测定人血清中拉莫三嗪(LTG)浓度的方法进行改进。方法:血清样品经甲醇处理后以高效液相色谱(HPLC)法进行测定,色谱柱为Hypersil BDS C18(200 mm×4.6 mm,5μm),流动相为甲醇-KH2PO4(45∶55,V/V),检测波长为250 nm,流速为1ml/min,柱温为35℃。结果:LTG血药浓度在0.5~30.0μg/ml范围内线性关系良好(r=0.999 9),日内、日间RSD均5%,方法回收率为101.96%~102.74%。结论:改进后的方法简便、快速、准确,适合临床常规监测LTG血药浓度及LTG的药动学研究。
[Abstract]:Objective: to improve the method for the determination of LTG in human serum. Methods: the serum samples were treated with methanol and determined by high performance liquid chromatography (HPLC). The chromatographic column was Hypersil BDS C18200mm 脳 4.6mm ~ 5 渭 m 路m ~ (-1), the mobile phase was methanol-KH _ 2PO _ 4: 4555 V / V, the detection wavelength was 250nm, the flow rate was 1ml / min, and the column temperature was 35 鈩，

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