海洋抑菌放线菌Y15的筛

发布时间：2018-03-18 09:35

本文选题：海洋抑菌放线菌　切入点：代谢产物　出处：《微生物学通报》2017年03期 　论文类型：期刊论文

【摘要】：【目的】筛选产广谱、高效抑菌活性物质的海洋放线菌,为新型抗生素的开发奠定基础。【方法】采用琼脂块法初筛,打孔扩散法复筛,以金黄色葡萄球菌、大肠埃希氏菌、枯草芽孢杆菌和单核细胞增生李斯特菌为指示菌从海泥样品中筛选目标菌株;利用20种指示菌株考查Y10、Y11、Y15、Y16和Y21的抑菌谱;通过形态观察和16S r RNA基因序列分析确定菌株分类地位;以单核细胞增生李斯特菌的OD600值为指标探讨Y15抑菌活性物质对该菌的作用方式;以抑菌活性为指标研究Y15抑菌活性物质的理化性质。【结果】共分离12株具抑菌活性的海洋放线菌,其中Y15抑菌谱最广,对20种指示菌株中的18种具有抑菌活性,并且在4种培养基中均能产生抑菌活性物质。16S r RNA基因序列分析表明Y15属于小单孢菌属,并与Micromonospora endolithica亲缘关系最近。Y15抑菌活性物质在144 h达到最高值为480 AU/m L,在168-216 h保持平衡为320 AU/m L。Y15抑菌活性物质对单核细胞李斯特菌作用方式为杀菌。Y15抑菌活性物质在-20-60°C抑菌活性保持稳定,在80-120°C活性逐渐下降,但在120°C处理30 min仍保留37.5%的活性;在p H 7.0-10.0抑菌活性稳定,在p H 2.0-6.0和p H 11.0-12.0抑菌活性均有所损失;Y15抑菌活性物质对紫外和4种酶(蛋白酶K、胰蛋白酶、木瓜蛋白酶和α-淀粉酶)处理均保持稳定,表明活性物质为非蛋白和非多肽类的抑菌物质。【结论】Y15产生的活性物质具有良好的抑菌谱和抑菌活性,且较为稳定,具有较高的应用价值。
[Abstract]:[objective] to screen marine actinomycetes producing broad-spectrum and high effective antibacterial active substances, and to lay the foundation for the development of new antibiotics. [methods] Agar block method was used to screen, perforated diffusion method was used to screen again, Staphylococcus aureus and Escherichia coli were used. Bacillus subtilis and Listeria monocytogenes were used as indicators to screen the target strains from the sea mud samples, and the bacteriostatic spectra of Y10, Y11, Y15, Y16 and Y21 were examined, and the taxonomic status of the strains was determined by morphological observation and 16s r RNA gene sequence analysis. The OD600 value of Listeria monocytogenes was used as an index to study the effect of Y15 active substance on Listeria monocytogenes. The physical and chemical properties of bacteriostatic substances of Y15 were studied. [results] 12 strains of marine actinomycetes with antibacterial activity were isolated, among which Y15 had the most extensive antibacterial spectrum and had antibacterial activity against 18 of the 20 indicating strains. The sequence analysis of 16s r RNA gene showed that Y15 belongs to the genus Micromonospora. The most recent phylogenetic relationship with Micromonospora endolithica was that the bacteriostatic activity of .Y15 reached a maximum value of 480 AU/m / L at 144h, and kept a balance at 168-216h for 320 AU/m L.Y15 antibacterial substances acting on Listeria monocytogenes. The bacteriostatic activity of -20-60 掳C remained stable. The activity decreased gradually at 80-120 掳C, but remained at 37.5% at 120 掳C for 30 min, and remained stable at pH 7.0-10.0, and decreased in pH 2.0-6.0 and pH 11.0-12.0 for UV and four enzymes (protease K, trypsin). Both papain and 伪 -amylase) treatments remained stable, indicating that the active substances were non-protein and non-polypeptide antimicrobial substances. [conclusion] the active substances produced by Y15 have good bacteriostatic spectrum and antibacterial activity, and are more stable. It has high application value.
【作者单位】：上海海洋大学食品学院上海水产品加工及贮藏工程技术研究中心上海海洋大学食品热加工工程技术研究中心;
【基金】：上海市科学技术委员会工程中心建设项目(No.11DZ2280300)~~
【分类号】：R915

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