双膦酸盐类药物对胃癌细胞生长增殖的作用机制

发布时间：2018-03-18 15:14

本文选题：双膦酸盐类药物　切入点：唑来膦酸　出处：《中国老年学杂志》2017年14期 　论文类型：期刊论文

【摘要】：目的检测双膦酸盐类药物唑来膦酸对胃癌细胞生长增殖的研究机制。方法培养人胃癌细胞株MKN-28、SGC-7901和BGC-823,将细胞分为对照组和药物处理组,MTT法检测10、20、40、80μmol/L唑来膦酸对细胞株的影响。人胃癌细胞株加入10~80μmol/L的唑来膦酸,对照组不加药物,孵育1、3、6、12、24 h后计算不同浓度和时间对细胞的影响;人胃癌细胞株SGC-7901加入40μmol/L的唑来膦酸培养24 h后,用流式细胞仪检测细胞凋亡情况,Western印迹检测半胱氨酸蛋白酶(Caspase)-3、Caspase-9、Bcl-2蛋白的表达。结果 10、20、40、80μmol/L的唑来膦酸作用于胃癌细胞株(BGC-823、SGC-7901、MKN-28),唑来膦酸的半抑制浓度(IC50)为40μmol/L,用10~80μmol/L的唑来膦酸作用于BGC-823、SGC-7901、MKN-28胃癌细胞株1、3、6、12、24 h后,可见唑来膦酸抑制人胃癌细胞的生长且有时间和浓度的依赖性,唑来膦酸对胃癌正常黏膜细胞GES-1无抑制作用;人胃癌细胞SGC-7901经40μmol/L的唑来膦酸作用24 h后,流式细胞仪检测发现唑来膦酸处理组细胞凋亡情况显著高于对照组;经40μmol/L唑来膦酸作用后的人胃癌细胞中Caspase-3、Caspase-9蛋白表达量明显升高,而Bcl-2蛋白表达量与对照组相比明显减少。结论唑来膦酸能抑制胃癌细胞的生长且有时间和剂量依赖性,唑来膦酸可以促进细胞凋亡。
[Abstract]:Objective to investigate the effect of zoledronic acid, a bisphosphonate drug, on the growth and proliferation of gastric cancer cells. Methods Human gastric cancer cell lines MKN-28, SGC-7901 and BGC-823 were cultured. The cells were divided into two groups: control group and drug treated group. MTT assay was used to detect the effect of zoledronic acid on gastric cancer cell line MKN-28SG-7901 and BGC-823. Human gastric cancer cell line was added with zoledronic acid of 10 ~ 80 渭 mol/L, In the control group, the effect of different concentration and time on the cells was calculated after incubation for 24 h, and the human gastric cancer cell line SGC-7901 was incubated with 40 渭 mol/L zoledronic acid for 24 h. Flow cytometry was used to detect cell apoptosis and Western blot was used to detect the expression of caspase-9 protein in human gastric cancer cell line BGC-823SG-7901MKN-281.The semi-inhibitory concentration of zoledronic acid was 40 渭 mol / L, and that of zoledronic acid was 40 渭 mol / L with 1080 渭 mol/L zoledronic acid. The acid treated BGC-823 SGC-7901 MKN-28 gastric cancer cell line 1D3, MKN-28 for 24 h, and the acid was used in the treatment of BGC-823 SGC-7901 MKN-28 gastric cancer cell line. It can be seen that zoledronic acid inhibits the growth of human gastric cancer cells in a time-and concentration-dependent manner, and zoledronic acid has no inhibitory effect on GES-1 of normal gastric cancer cells, and SGC-7901 is treated with 40 渭 mol/L zoledronic acid for 24 h. The results of flow cytometry showed that the apoptosis of the cells treated with zoledronic acid was significantly higher than that of the control group, and the expression of Caspase-3 and Caspase-9 protein in gastric cancer cells treated with 40 渭 mol/L zoledronic acid was significantly higher than that in the control group. Conclusion Zoledronic acid can inhibit the growth of gastric cancer cells in a time-and dose-dependent manner, and zoledronic acid can promote the apoptosis of gastric cancer cells.
【作者单位】：青海大学附属医院肿瘤内科;
【分类号】：R96

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