维生素C对青春期邻苯二甲酸二丁酯暴露致大鼠卵巢氧化应激的干预作用

发布时间：2018-03-18 15:38

本文选题：邻苯二甲酸二丁酯　切入点：维生素C　出处：《环境与健康杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的探讨维生素C(vitamin C,Vit C)对青春期邻苯二甲酸二丁酯(dibutyl phthalate,DBP)暴露致大鼠卵巢氧化应激的干预作用。方法将160只健康初断乳SPF级Wistar雌性大鼠随机分为5组,分别为对照(玉米油)组和低(100mg/kg)、高(500 mg/kg)剂量DBP暴露组及低(100 mg/kg)、高(500 mg/kg)剂量DBP+Vit C(125 g/L)干预组。采用灌胃方式暴露DBP,暴露容量为10 ml/kg,每天1次;采用自由饮水方式暴露Vit C,连续暴露30 d。分别于暴露第5、10、20、30天,测定大鼠卵巢组织中MDA、GSH含量和SOD、CAT、GSH-Px活力。结果与对照组相比,DBP暴露组大鼠卵巢组织中MDA含量均升高,而GSH含量和SOD、CAT、GSH-Px活力均下降;与相同剂量DBP暴露组相比,Vit C干预组大鼠卵巢组织中MDA均下降,而GSH含量和SOD、CAT、GSH-Px活力均升高。且随着DBP暴露剂量的升高,DBP暴露组和Vit C干预组大鼠卵巢组织中的GSH含量和SOD、CAT、GSH-Px活力均呈下降趋势,而MDA含量呈上升趋势。结论 DBP暴露可致青春期雌性大鼠卵巢组织产生脂质过氧化反应,诱导氧化应激,导致卵巢抗氧化能力下降;而抗氧化剂Vit C对于DBP所致生殖系统的氧化损伤具有一定的拮抗作用。
[Abstract]:Objective to investigate the effect of vitamin C vitamin C (Vit C) on oxidative stress induced by dibutyl phthalate dibutyl phthalate (DBP) in pubertal rats. Methods 160 healthy SPF female rats were randomly divided into 5 groups. They were the control group (corn oil), the low dose of DBP (100mg / kg), the low dose of DBP (100mg / kg) and the group of low dose (100mg / kg) DBP Vit (125g / L). The exposure capacity was 10ml / kg, once a day, and the dose of DBP Vit (125g / L) was 100 mg / kg, 100 mg / kg, 100 mg / kg, 100 mg / kg, 100 mg / kg, 100 mg / kg, 100 mg / kg, 100 mg / kg, 100 mg / kg respectively, respectively. Vit C was exposed to free drinking water for 30 days. The content of MDA-GSH and the activity of MDA in ovarian tissue of rats were measured on the 20th day after exposure. Results compared with the control group, the content of MDA in the ovarian tissue of the rats exposed to Vit was higher than that of the control group. The content of GSH and the activity of GSH-Px in DBP were decreased, and the MDA in ovarian tissue of Vit C group was decreased compared with the same dose of DBP exposure group. However, the content of GSH and the activity of GSH-Px in DBP increased, and the content of GSH and the activity of GSH-Px in the ovary of rats exposed to DBP and treated with Vit C showed a decreasing trend with the increase of DBP exposure dose. Conclusion DBP exposure can induce lipid peroxidation in the ovary of adolescent female rats, induce oxidative stress and decrease the antioxidant capacity of ovary. The antioxidant Vit C has a certain antagonistic effect on the oxidative damage of reproductive system induced by DBP.
【作者单位】：衢州职业技术学院医学院;
【基金】：浙江省公益性技术研究项目(2016C33239) 衢州职业技术学院科研项目基金(QZYY1517) 衢州市科技计划项目(2016Y08)
【分类号】：R965

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