小鼠垂体中叶素对异丙肾上腺素诱导的小鼠心脏肥大的保护作用研究

发布时间：2018-03-21 20:09

本文选题：异丙肾上腺素　切入点：心脏扩大　出处：《重庆医学》2017年01期 　论文类型：期刊论文

【摘要】：目的利用异丙肾上腺素(ISO)诱导的小鼠心脏肥大模型研究小鼠垂体中叶素(IMD)拮抗心脏肥大的药理学作用和机制。方法建立小剂量ISO皮下注射诱导心脏肥大的小鼠模型,研究IMD给药后的小鼠血流动力学指标和心质量/体质量比,然后利用心脏组织切片评价心肌细胞横截面积、凋亡和纤维化。采用荧光实时定量PCR法检测心脏组织的心钠素(ANP)、脑钠肽(BNP)、内源性IMD及其受体系统的mRNA表达水平。结果与ISO诱导的小鼠心脏肥大模型组相比较,腹腔注射IMD治疗明显降低ISO处理所增加的心质量/体质量比和心肌细胞横截面积、心脏肥大标志物ANP及BNP的mRNA水平,以及心肌细胞凋亡率和心肌组织纤维化率。同时,改善心脏功能,左室压力、等容收缩期左心室内压力上升的最大和下降的最小速率、每搏排血量、心输出量和射血分数明显增加,而左心室舒张末期压力显著降低。此外,ISO处理明显诱导心脏组织内源性IMD及其受体的表达。结论 ISO诱导心脏组织内源性IMD及其受体的表达,外源性给与IMD治疗可能通过ISO活化的IMD受体系统实现其拮抗心脏肥大的药理学保护作用。
[Abstract]:Objective to study the pharmacological effect and mechanism of isoprenaline (ISO) -induced cardiac hypertrophy in mice. Methods the mice model of cardiac hypertrophy induced by subcutaneous injection of low dose ISO was established. The hemodynamic indexes and heart mass / body mass ratio of mice treated with IMD were studied, and then the cross sectional area of cardiomyocytes was evaluated by cardiac tissue sections. Apoptosis and fibrosis. The mRNA expression levels of atrial natriuretic peptide (ANPN), brain natriuretic peptide (BNPN), endogenous IMD and its receptor system in heart tissue were detected by fluorescence real-time quantitative PCR. The results were compared with those in ISO induced cardiac hypertrophy model group. Intraperitoneal injection of IMD significantly decreased the increased cardiac mass / body mass ratio, myocardial cell cross-sectional area, mRNA levels of cardiac hypertrophy markers ANP and BNP, myocardial apoptosis rate and myocardial fibrosis rate. Improvement of cardiac function, left ventricular pressure, maximum and minimum rate of decrease of isovolumic systolic pressure in left ventricle, significant increase in cardiac output, cardiac output and ejection fraction per stroke, In addition, the expression of endogenous IMD and its receptors in cardiac tissue was significantly induced by IMD. Conclusion ISO induces the expression of endogenous IMD and its receptors in cardiac tissue. Exogenous IMD therapy may achieve pharmacological protection against cardiac hypertrophy through ISO activated IMD receptor system.
【作者单位】：四川省人民医院感染内科;四川大学华西医院分子医学研究中心;
【基金】：国家自然科学基金面上项目(31071001,31271226)
【分类号】：R965

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