N-双嘧啶取代芳胺的合成及其抗氧化损伤研究

发布时间：2018-03-23 17:34

  本文选题：N-双嘧啶取代芳胺衍生物　切入点：双氧水　出处：《化学试剂》2017年10期

【摘要】：报道了一种N-双嘧啶取代芳胺衍生物2-(3-氯苯基)-N-(2-(3-氯苯基)-6-甲基嘧啶-4-基)-N-(2-甲氧苯基)-6-甲基嘧啶-4-胺的合成、表征及其抗氧化生物活性,该N-双嘧啶取代芳胺衍生物是以3-氯苄腈为原料经4步反应合成而得到,其结构通过1HNMR、13CNMR、HR-MS进行了表征和确认。化合物的抗氧化作用与细胞毒作用分别通过是否进行双氧水处理的SH-SY5Y细胞与CCK-8试剂盒进行检测,通过荧光探针DCFH-DA标记检测细胞内ROS含量。结果表明,该化合物具有抗双氧水氧化损伤的可能,能够显著提高细胞存活率且没有明显的细胞毒性。
[Abstract]:This paper reports the synthesis, characterization and antioxidant biological activity of a derivative of N-dipyrimidine substituted aryl amines (2-O3-chlorophenyl) -N-n- (3-chlorophenyl) -6-methylpyrimidine (4-methylpyrimidine) -6-methoxyphenyl (2-methoxyphenyl) -6-methylpyrimidine-4- (4-amine), which is a derivative of N-dipyrimidine substituted aryl amines. The N-dipyrimidine substituted aromatic amine derivative was synthesized from 3-chlorobenzylonitrile in four steps. Its structure was characterized and confirmed by 1H-NMR-13CNMR-HR-MS. The antioxidation and cytotoxicity of the compounds were detected by SH-SY5Y cells treated with hydrogen peroxide and CCK-8 kit, respectively. Fluorescence probe DCFH-DA labeling was used to detect the content of ROS in the cells. The results showed that the compound had the possibility of resisting oxidative damage of hydrogen peroxide and could significantly improve the cell survival rate without obvious cytotoxicity.
【作者单位】： 武汉科技大学化学与化工学院;武汉科技大学生物医学研究院;
【基金】：湖北省自然科学基金资助项目(2015CFB490)
【分类号】：R914
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本文编号：1654474