二十二碳六烯酸对豚鼠顺铂所致耳毒性的影响

发布时间：2018-03-24 02:23

本文选题：顺铂　切入点：二十二碳六烯(DHA)　出处：《重庆医学》2015年09期

【摘要】：目的通过动物实验,研究二十二碳六烯(DHA)全身给药对顺铂所致豚鼠耳毒性的保护作用。方法将健康豚鼠24只分为正常对照组、DHA加顺铂组、顺铂组,DHA加顺铂组连续给予植物油稀释后的DHA灌胃,500 mg·kg-1·d-1,共30d。在灌胃22d时,开始生理盐水稀释后的0.25mg/mL的顺铂溶液腹腔注射,连续9d,正常对照组和顺铂组只给予相同剂量植物油灌胃。结果顺铂造模后,顺铂组和DHA加顺铂组ABR阈值明显高于正常对照组,DHA加顺铂组明显低于顺铂组。DHA加顺铂组外毛细胞残存数明显高于顺铂组,组织形态优于顺铂组,螺旋神经节细胞形态顺铂组差于DHA加顺铂组。结论 DHA灌胃给药对临床顺铂化疗耳毒性并发症有一定的保护作用。
[Abstract]:Objective to study the protective effect of 22 carbohexene on guinea pig ototoxicity induced by cisplatin. Methods 24 healthy guinea pigs were divided into normal control group (n = 24) and cisplatin group (n = 24). In the cisplatin group, the DHA plus cisplatin group was continuously given 500mg kg-1 d -1 of DHA diluted with vegetable oil for 30 days. After 22 days of administration, the cisplatin solution of 0.25mg/mL diluted with normal saline was injected intraperitoneally. For 9 days, the normal control group and the cisplatin group were given only the same dose of vegetable oil. The ABR threshold of cisplatin group and DHA plus cisplatin group was significantly higher than that of normal control group. The number of outer hair cells in cisplatin group was significantly higher than that in cisplatin group, and the tissue morphology was better than that in cisplatin group. The morphology of spiral ganglion cells was worse than that of DHA plus cisplatin group. Conclusion the oral administration of DHA has a protective effect on ototoxic complications of clinical cisplatin chemotherapy.
【作者单位】：成都大学医护学院解剖教研室;四川大学第二附属医院生殖内分泌科;
【基金】：2011年成都大学校基金资助项目(2011xj219) 2012年四川省卫生厅自然科学基金资助项目(120584)
【分类号】：R965

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