丹酚酸A对异丙肾上腺素致小鼠心肌缺血的保护作用及其机制

发布时间：2018-03-24 04:38

本文选题：丹酚酸A　切入点：异丙肾上腺素　出处：《中国药理学通报》2015年12期

【摘要】：目的丹酚酸A是我国传统中药丹参中提取的主要水溶性成分之一,本研究探讨了丹酚酸A抗异丙肾上腺素诱导的小鼠心肌缺血的作用及其机制。方法采用皮下注射异丙肾上腺素(isopropranol,ISO)8 mg·kg~(-1)致小鼠心肌缺血模型,从动物死亡率、心电图、心脏指数、血清心肌酶谱以及病理切片评价丹酚酸A对小鼠心肌的保护作用,并从抗氧化、炎症以及细胞凋亡通路探讨其作用机制。结果丹酚酸A能够剂量依赖性地增强心肌缺血小鼠的心脏功能,减少心肌损伤酶的漏出,改善缺血心肌的病理变化,具有明显的心肌保护作用。丹酚酸A能降低心肌炎症因子白细胞介素-6(interleukin-6,IL-6)、肿瘤坏死因子-α(tumornecrosis factor-α,TNF-α)的水平,减少ISO损伤引起的心肌细胞凋亡,增加抗凋亡蛋白Bcl-2表达,降低促凋亡蛋白Bax表达,并上调Akt的磷酸化水平。结论丹酚酸A具有明显的抗心肌缺血作用,其机制可能与稳定机体抗氧化系统,抑制炎症因子生成,以及调节凋亡蛋白表达及PI3K/Akt细胞凋亡信号通路有关。
[Abstract]:Objective Salvianolic acid A is one of the main water-soluble components extracted from traditional Chinese medicine Salvia miltiorrhiza. The purpose of this study was to investigate the protective effect of Salvianolic acid A on isoprenaline induced myocardial ischemia in mice and its mechanism. Methods the myocardial ischemia model was induced by subcutaneous injection of isopropranolol isopropranolol (ISO 8 mg 路kg ~ (-1)) in mice. The protective effect of Salvianolic acid A on myocardium of mice was evaluated by serum myocardial enzyme spectrum and pathological sections. Results Salvianolic acid A could enhance the heart function of myocardial ischemia mice in a dose-dependent manner, reduce the leakage of myocardial injury enzymes, and improve the pathological changes of ischemic myocardium. Salvianolic acid A can reduce the level of interleukin-6tr, tumor necrosis factor- 伪 (TNF- 伪), decrease the apoptosis of cardiomyocytes induced by ISO injury, and increase the expression of anti-apoptotic protein (Bcl-2). Conclusion Salvianolic acid A has obvious anti-myocardial ischemia effect, and its mechanism may be to stabilize the antioxidant system and inhibit the production of inflammatory factors. And regulation of apoptotic protein expression and PI3K/Akt cell apoptosis signal pathway.
【作者单位】：中国医学科学院北京协和医学院药物研究所北京市药物靶点研究与新药筛选重点实验室;
【基金】：国家科技部重大专项“重大新药创制”(No 2013ZX091030-01-008,2012ZX09103101-078,2013ZX09508104) 国家自然科学基金面上项目(No 81573645)
【分类号】：R965

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