牛膝有效部位在肿瘤微环境下促进树突状细胞成熟及功能调控的研究

发布时间：2018-03-25 08:44

本文选题：牛膝　切入点：树突状细胞　出处：《中国药学杂志》2015年23期

【摘要】：目的观察牛膝有效部位在肿瘤微环境下对树突状细胞成熟的促进及功能调控作用。方法干燥牛膝粉末提取并鉴定牛膝总皂苷和牛膝多糖;建立模拟肿瘤微环境的MCF-7乳腺癌细胞与树突状细胞共培养体系,并分别给予牛膝多糖、牛膝总皂苷及牛膝多糖和总皂苷协同刺激;流式细胞仪检测树突状细胞表面分子的表达及树突状细胞诱导的细胞毒性T淋巴细胞(CTL)杀伤活性,ELISA法检测培养上清中白细胞介素-1β、肿瘤坏死因子-α、白细胞介素-10和白细胞介素-12的水平,Western blot检测JAK/STAT3信号通路中p-JAK、JAK、p-STAT3、STAT3的蛋白表达。结果在肿瘤微环境下,牛膝有效部位牛膝总皂苷和牛膝多糖均可以通过TLR4及Dectin-1受体途径,启动树突状细胞细胞JAK/STAT3信号通路,上调树突状细胞细胞表面分子CD80、CD86及CD40的表达,从而诱导树突状细胞的成熟与分化,促进肿瘤坏死因子-α、白细胞介素-12、白细胞介素-10及白细胞介素-1β等细胞因子的分泌,两者协同较单独应用作用加强。结论以适当形式的中药有效部位作为免疫增强剂联合树突状细胞疫苗,可有效纠正肿瘤微环境下的免疫缺陷,有效激活肿瘤特异性CTL细胞,从而产生特异性抗肿瘤效应。
[Abstract]:Objective to observe the effects of active fractions of Achyranthes bidentata on the maturation and function of dendritic cells in tumor microenvironment. Methods the total saponins and polysaccharides of Achyranthes bidentata were extracted and identified by dried powder of Achyranthes bidentata. To establish a co-culture system of MCF-7 breast cancer cells and dendritic cells simulated by tumor microenvironment, and give them co-stimulation of Achyranthes bidentata polysaccharides, total saponins, Achyranthes bidentata polysaccharides and total saponins, respectively. Flow cytometry was used to detect the expression of dendritic cell surface molecules and the cytotoxic T lymphocyte CTL cytotoxicity induced by dendritic cells. Elisa was used to detect interleukin-1 尾, tumor necrosis factor- 伪 and interleukin-10 in the culture supernatant. The levels of IL-12 and IL-12 were measured by Western blot to detect the protein expression of p-JAKT3-STAT3STAT3 in the JAK/STAT3 signaling pathway. The total saponins of Achyranthes bidentata and polysaccharides of Achyranthes bidentata can activate the JAK/STAT3 signaling pathway of dendritic cells through TLR4 and Dectin-1 receptor pathway and up-regulate the expression of CD80 CD86 and CD40 on the surface of dendritic cells. Thus inducing the maturation and differentiation of dendritic cells and promoting the secretion of cytokines such as tumor necrosis factor- 伪, interleukin-12, interleukin-10 and interleukin-1 尾. Conclusion the effective part of traditional Chinese medicine combined with dendritic cell vaccine can effectively correct the immune deficiency in tumor microenvironment and activate tumor specific CTL cells. Thus, the specific anti-tumor effect is produced.
【作者单位】：辽宁中医药大学;
【基金】：辽宁省教育厅科学研究一般项目资助项目(L2012331)
【分类号】：R96

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