氮氧自由基对高原缺氧小鼠脑组织中缺氧和凋亡蛋白的影响

发布时间：2018-03-25 08:45

本文选题：氮氧自由基　切入点：高原缺氧　出处：《中国医院药学杂志》2015年22期

【摘要】：目的:研究氮氧自由基HPN对高原缺氧小鼠脑组织中缺氧和凋亡蛋白的影响。方法:将60只小鼠随机分为正常对照组、缺氧模型组、乙酰唑胺组和HPN组,单次腹腔注射给药后,在模拟海拔8 000 m环境停留12 h,检测脑组织中乳酸脱氢酶(LD)含量和乳酸脱氢酶(LDH)活性的变化,HE染色观察脑组织病理学改变,蛋白印迹法检测HIF-1α、VEGF、Caspase-3、Bcl-2和Bax蛋白的表达情况。结果:与正常对照组相比,缺氧模型组中LD含量显著增加,LDH活性显著减低,HIF-1α、VEGF、Caspase-3和Bax蛋白表达增强,Bcl-2蛋白表达和Bcl-2/Bax比值降低。经HPN预处理后能够显著降低高原缺氧小鼠脑组织中LD含量,提高LDH活性,改善脑组织学变化,降低HIF-1α、VEGF、Caspase-3和Bax蛋白表达,提高Bcl-2的蛋白表达和Bc1-2/Bax比值。结论:HPN能够减轻高原缺氧脑组织损伤,作用机制可能与改善能量代谢,降低机体氧化应激,抑制某些凋亡相关蛋白表达有关。
[Abstract]:Objective: to study the effect of nitroxide free radical (HPN) on hypoxic and apoptotic protein in brain tissue of mice exposed to hypoxia at high altitude. Methods: 60 mice were randomly divided into normal control group, hypoxia model group, acetazolamide group and HPN group. The contents of lactate dehydrogenase (LDH) and the activity of lactate dehydrogenase (LDH) in brain tissue were measured for 12 hours in a simulated altitude of 8 000 m. The pathological changes of brain tissue were observed by HE staining. Western blot was used to detect the expression of Bcl-2 and Bax protein in HIF-1 伪 -VEGFN Caspase-3. Results: compared with the normal control group, the expression of Bcl-2 and Bax protein was detected by Western blotting. In hypoxia model group, LD content significantly increased and LDH activity decreased significantly. The expression of HIF-1 伪 VEGFU Caspase-3 and Bax protein increased and Bcl-2 protein expression and Bcl-2/Bax ratio decreased. After pretreatment with HPN, LD content and LDH activity in brain tissue of hypoxic mice at high altitude were significantly decreased. The changes of brain histology were improved, the expression of Caspase-3 and Bax protein of HIF-1 伪 -VEGF was decreased, the protein expression of Bcl-2 and the ratio of Bc1-2/Bax were increased. Conclusion the effect of HIF-1 伪 -VEGF can reduce the damage of hypoxic brain tissue at high altitude, which may be related to the improvement of energy metabolism and the reduction of oxidative stress. Inhibition of the expression of some apoptosis-related proteins.
【作者单位】：兰州军区兰州总医院药剂科全军高原环境损伤防治重点实验室;
【基金】：国家自然科学基金项目(编号:81202458) 全军医药科研“十二五”面上项目(编号:CLZ12JA04) 甘肃省自然科学基金(编号:1308RJYA06) 中国博士后科学基金(编号:2012M521926)
【分类号】：R965

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